PMID- 11359906
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20230413
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 21
IP  - 12
DP  - 2001 Jun
TI  - The alpha and beta subunits of IkappaB kinase (IKK) mediate TRAF2-dependent IKK 
      recruitment to tumor necrosis factor (TNF) receptor 1 in response to TNF.
PG  - 3986-94
AB  - The activation of IkappaB kinase (IKK) is a key step in the nuclear translocation 
      of the transcription factor NF-kappaB. IKK is a complex composed of three 
      subunits: IKKalpha, IKKbeta, and IKKgamma (also called NEMO). In response to the 
      proinflammatory cytokine tumor necrosis factor (TNF), IKK is activated after 
      being recruited to the TNF receptor 1 (TNF-R1) complex via TNF 
      receptor-associated factor 2 (TRAF2). We found that the IKKalpha and IKKbeta 
      catalytic subunits are required for IKK-TRAF2 interaction. This interaction 
      occurs through the leucine zipper motif common to IKKalpha, IKKbeta, and the RING 
      finger domain of TRAF2, and either IKKalpha or IKKbeta alone is sufficient for 
      the recruitment of IKK to TNF-R1. Importantly, IKKgamma is not essential for 
      TNF-induced IKK recruitment to TNF-R1, as this occurs efficiently in 
      IKKgamma-deficient cells. Using TRAF2(-/-) cells, we demonstrated that the 
      TNF-induced interaction between IKKgamma and the death domain kinase RIP is TRAF2 
      dependent and that one possible function of this interaction is to stabilize the 
      IKK complex when it interacts with TRAF2.
FAU - Devin, A
AU  - Devin A
AD  - Department of Cell and Cancer Biology, Medicine Branch, Division of Clinical 
      Sciences, National Cancer Institute, National Institutes of Health, Bethesda, 
      Maryland 20892, USA.
FAU - Lin, Y
AU  - Lin Y
FAU - Yamaoka, S
AU  - Yamaoka S
FAU - Li, Z
AU  - Li Z
FAU - Karin, M
AU  - Karin M
FAU - Liu Zg
AU  - Liu Zg
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Antigens, CD)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Protein Subunits)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (RIPK1 protein, human)
RN  - EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Ripk1 protein, mouse)
RN  - EC 2.7.11.10 (CHUK protein, human)
RN  - EC 2.7.11.10 (Chuk protein, mouse)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - EC 2.7.11.10 (IKBKB protein, human)
RN  - EC 2.7.11.10 (IKBKE protein, human)
RN  - EC 2.7.11.10 (Ikbkb protein, mouse)
RN  - EC 2.7.11.10 (Ikbke protein, mouse)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - Cell Line
MH  - Humans
MH  - I-kappa B Kinase
MH  - Leucine Zippers
MH  - Macromolecular Substances
MH  - Mice
MH  - Protein Serine-Threonine Kinases/*chemistry/genetics/*metabolism
MH  - Protein Subunits
MH  - Proteins/genetics/*metabolism
MH  - Rats
MH  - Receptor-Interacting Protein Serine-Threonine Kinases
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - Receptors, Tumor Necrosis Factor, Type I
MH  - TNF Receptor-Associated Factor 2
MH  - Transfection
MH  - Tumor Necrosis Factor-alpha/*pharmacology
PMC - PMC87061
EDAT- 2001/05/22 10:00
MHDA- 2001/06/29 10:01
PMCR- 2001/06/01
CRDT- 2001/05/22 10:00
PHST- 2001/05/22 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/05/22 10:00 [entrez]
PHST- 2001/06/01 00:00 [pmc-release]
AID - 2046 [pii]
AID - 10.1128/MCB.21.12.3986-3994.2001 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2001 Jun;21(12):3986-94. doi: 10.1128/MCB.21.12.3986-3994.2001.