PMID- 11360276
OWN - NLM
STAT- MEDLINE
DCOM- 20010802
LR  - 20101118
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 21
IP  - 5
DP  - 2001 May
TI  - Magnetic-activated cell sorting (MACS) significantly decreases the hybridization 
      efficiency of fluorescence in situ hybridization (FISH).
PG  - 359-61
AB  - Fetal cells were enriched from maternal blood using density gradient 
      centrifugation of Histopaque followed by magnetic-activated cell sorting (MACS) 
      to select CD71-positive cells. For each specimen, cells partially purified by 
      Histopaque were split into equal portions, and each portion was subjected to 
      purification by MACS in parallel. Cells before and after MACS were subjected to 
      dual-color fluorescence in situ hybridization (FISH) analysis with X- and 
      Y-chromosome-specific probes. We found that the hybridization rates were 
      decreased by approximately 10% after MACS based on duplicated analysis for each 
      sample.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Kuo, P L
AU  - Kuo PL
AD  - Department of Obstetrics & Gynecology, National Cheng-Kung University Medical 
      College, Tainan, Taiwan, ROC. paolink@mail.ncku.edu.tw
FAU - Guo, H R
AU  - Guo HR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - Cell Separation
MH  - Female
MH  - Fetal Blood/*cytology/immunology
MH  - Humans
MH  - *Immunomagnetic Separation
MH  - In Situ Hybridization, Fluorescence/*methods/standards
MH  - Male
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Reproducibility of Results
MH  - Sex Determination Analysis
EDAT- 2001/05/22 10:00
MHDA- 2001/08/03 10:01
CRDT- 2001/05/22 10:00
PHST- 2001/05/22 10:00 [pubmed]
PHST- 2001/08/03 10:01 [medline]
PHST- 2001/05/22 10:00 [entrez]
AID - 10.1002/pd.71 [doi]
PST - ppublish
SO  - Prenat Diagn. 2001 May;21(5):359-61. doi: 10.1002/pd.71.