PMID- 11360297
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20191104
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 34
IP  - 4
DP  - 2001 Jun
TI  - An immortalized jimpy oligodendrocyte cell line: defects in cell cycle and cAMP 
      pathway.
PG  - 241-52
AB  - Normal and jimpy oligodendrocytes in secondary cultures were transfected with 
      plasmids containing the SV40 T-antigen gene expressed under the control of the 
      mouse metallothionein-I promoter. Two immortalized stable cell lines, a normal 
      (158N) and jimpy (158JP) cell line, expressed transcripts and proteins of 
      oligodendrocyte markers, including proteolipid protein (PLP), myelin basic 
      protein (MBP), and carbonic anhydrase II (CAII). Galactocerebroside and sulfatide 
      were also detected with immunocytochemistry. Immunoelectron microscopy using gold 
      particles showed that the truncated endogenous jimpy PLP was distributed 
      throughout the cytoplasm and in association with the plasma membrane of cell 
      bodies and processes. The length of the cell cycle in the jimpy oligodendrocytes 
      in the absence of zinc was 31 h, about a 4-h longer cell cycle than the normal 
      line. In the presence of 100 microM zinc, the cell cycle became 3 h shorter for 
      both cell lines, with the jimpy cell cycle duration remaining 4 h longer than the 
      normal line. Interestingly, the jimpy cell line showed a significant deficiency 
      in stimulation via the cAMP pathway. While the level of oligodendrocyte markers 
      (PLP, MBP, and CAII) were significantly increased by dibutyryl cAMP (dbcAMP) 
      treatment in the normal cell line, no changes were observed in the jimpy cell 
      lines. This observation, together with previous results showing jimpy 
      oligodendrocyte's failure to respond to basic fibroblast growth factor (bFGF), 
      suggests a role for PLP in a signal transduction pathway. Jimpy and normal 
      oligodendrocytes transfected with the SV40T antigen gene, driven by the wild-type 
      promoter of mouse metallothionein-I, continue to express properties of 
      oligodendrocytes and therefore provide a powerful model to explore the function 
      of myelin proteins and to dissect the complexity of the jimpy phenotype.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Feutz, A C
AU  - Feutz AC
AD  - CNRS-ER 2072, Institut de Chimie Biologique, Faculte de Medecine, Strasbourg, 
      France.
FAU - Pham-Dinh, D
AU  - Pham-Dinh D
FAU - Allinquant, B
AU  - Allinquant B
FAU - Miehe, M
AU  - Miehe M
FAU - Ghandour, M S
AU  - Ghandour MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Antigens, Viral, Tumor)
RN  - 0 (Biomarkers)
RN  - 0 (Myelin Proteolipid Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Plp1 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 63X7MBT2LQ (Bucladesine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Antigens, Viral, Tumor/metabolism
MH  - Biomarkers
MH  - Bromodeoxyuridine/metabolism
MH  - Bucladesine/pharmacology
MH  - Cell Cycle
MH  - Cell Division
MH  - Cell Line, Transformed
MH  - Cells, Cultured
MH  - Cyclic AMP/*metabolism
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Jimpy/*physiology
MH  - Microscopy, Electron
MH  - Myelin Proteolipid Protein/*genetics
MH  - *Nerve Tissue Proteins
MH  - Neurons/cytology/physiology
MH  - Oligodendroglia/*cytology/drug effects/*metabolism/ultrastructure
MH  - RNA, Messenger/metabolism
MH  - Reference Values
MH  - Tissue Distribution
EDAT- 2001/05/22 10:00
MHDA- 2001/07/13 10:01
CRDT- 2001/05/22 10:00
PHST- 2001/05/22 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/05/22 10:00 [entrez]
AID - 10.1002/glia.1058 [pii]
AID - 10.1002/glia.1058 [doi]
PST - ppublish
SO  - Glia. 2001 Jun;34(4):241-52. doi: 10.1002/glia.1058.