PMID- 11368004
OWN - NLM
STAT- MEDLINE
DCOM- 20010607
LR  - 20131121
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 385
IP  - 2
DP  - 2001 Jan 15
TI  - Localization, transport, and uptake of D-aspartate in the rat adrenal and 
      pituitary glands.
PG  - 242-9
AB  - Large amounts of D-aspartate (D-Asp) are present in the rat adrenal and pituitary 
      glands. D-Asp is thought to be synthesized in the mammalian body and also 
      accumulates in various tissues following intraperitoneal or intravenous 
      administration. This report examines the origins of D-Asp in the adrenal and 
      pituitary glands. We administered D-Asp to male rats intraperitoneally and 
      immunolocalized this exogenous D-Asp in adrenal and pituitary tissue, using an 
      anti-D-Asp antiserum which was previously developed in our laboratory. D-Asp 
      levels in the rat adrenal gland have been shown to undergo a transient increase 
      at 3 weeks of age and to decrease rapidly thereafter. We found that in the 
      adrenal gland, exogenous D-Asp administered intraperitoneally was incorporated 
      into the same region of the adrenal cortex in which endogenous D-Asp was present. 
      By Northern and Western blot analysis and immunohistochemistry of glutamate (Glu) 
      transporter, we also found that expression of the Glu transporter (GLAST), which 
      has an affinity for D-Asp, transiently increased at 3 weeks of age and that 
      localization patterns of the Glu transporter within the tissue were almost 
      coincident with those of endogenous D-Asp. These observations suggest that D-Asp 
      in the adrenal cortex of 3-week-old male rats is primarily acquired by uptake 
      from the vascular system. We have previously shown that D-Asp is specifically 
      localized in prolactin (PRL)-containing cells in the anterior lobe of the adult 
      rat pituitary gland. Here we report that in the pituitary gland, exogenous D-Asp 
      accumulated in endothelial cells, but not in PRL-containing cells. Northern and 
      Western blot analysis and immunohistochemistry of Glu transporter revealed that 
      developmental changes in the Glu transporter (GLAST) expression did not correlate 
      with tissue levels of D-Asp and that the Glu transporter was not expressed in 
      PRL-containing cells. These observations suggest that, in contrast to the adrenal 
      gland, most of the D-Asp in the pituitary gland of adult male rats originates 
      inside the gland itself.
FAU - Lee, J A
AU  - Lee JA
AD  - Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
FAU - Long, Z
AU  - Long Z
FAU - Nimura, N
AU  - Nimura N
FAU - Iwatsubo, T
AU  - Iwatsubo T
FAU - Imai, K
AU  - Imai K
FAU - Homma, H
AU  - Homma H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - 30KYC7MIAI (Aspartic Acid)
SB  - IM
MH  - Adrenal Glands/cytology/*metabolism
MH  - Animals
MH  - Aspartic Acid/administration & dosage/*metabolism
MH  - Biological Transport
MH  - Endothelium/cytology/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Immunohistochemistry
MH  - Injections, Intraperitoneal
MH  - Injections, Intravenous
MH  - Male
MH  - Monosaccharide Transport Proteins/genetics/metabolism
MH  - Pituitary Gland, Anterior/cytology/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2001/05/23 10:00
MHDA- 2001/06/08 10:01
CRDT- 2001/05/23 10:00
PHST- 2001/05/23 10:00 [pubmed]
PHST- 2001/06/08 10:01 [medline]
PHST- 2001/05/23 10:00 [entrez]
AID - S0003-9861(00)92163-5 [pii]
AID - 10.1006/abbi.2000.2163 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2001 Jan 15;385(2):242-9. doi: 10.1006/abbi.2000.2163.