PMID- 11369868
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20200217
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 82
IP  - Pt 6
DP  - 2001 Jun
TI  - Increased proteolytic activity and matrix metalloprotease expression in lungs 
      during infection by porcine reproductive and respiratory syndrome virus.
PG  - 1253-1261
LID - 10.1099/0022-1317-82-6-1253 [doi]
AB  - The local increase in the secretion of extracellular proteases, allowing cleavage 
      of the extracellular matrix and thereby facilitating the infiltration of T cells, 
      monocytes and neutrophils, is a hallmark of chronic inflammation and 
      autoimmunity. In pulmonary genetic diseases, such as emphysema and cystic 
      fibrosis, proteases can also favour the development of local immunodeficiency by 
      degrading key regulators of the immune response, such as CD4, CD8, IgG, ICAM-1 
      and C3b receptors. Since several infectious agents can give rise to severe 
      pulmonary disorders associated with opportunistic infections, we sought to 
      determine whether an increase in proteolytic activity occurred during infection 
      with porcine reproductive and respiratory syndrome virus (PRRSV), the causative 
      agent of a new disease in swine characterized by severe respiratory problems in 
      young pigs. Piglets were infected with the virus and bronchoalveolar lavages were 
      collected at various times post-infection to measure the net proteolytic 
      activity. It was shown that PRRSV infection leads to a significant increase in 
      proteolytic activity in pulmonary fluids. Maximal activity was found at 7 and 14 
      days post-infection, with a return towards normal levels at day 42. Zymographic 
      analyses showed a significant increase in the secretion of matrix 
      metalloproteases (MMPs) 2 and 9, two enzymes involved in tissue remodelling. 
      Histological analyses showed a correlation between the increase in proteolytic 
      activity and the appearance of lesions that were characterized by massive 
      lymphomononuclear cell infiltration. These results suggest that virus infection 
      of the lungs can lead to a transient increase in proteolytic activity that could 
      favour opportunistic infection.
FAU - Girard, Manon
AU  - Girard M
AD  - Centre de Recherche en Sante Humaine1 et Centre de Recherche en Microbiologie et 
      Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, 
      Laval-des-Rapides, Quebec, CanadaH7N 4Z3.
FAU - Cleroux, Patrick
AU  - Cleroux P
AD  - Centre de Recherche en Sante Humaine1 et Centre de Recherche en Microbiologie et 
      Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, 
      Laval-des-Rapides, Quebec, CanadaH7N 4Z3.
FAU - Tremblay, Pierre
AU  - Tremblay P
AD  - Centre de Recherche en Sante Humaine1 et Centre de Recherche en Microbiologie et 
      Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, 
      Laval-des-Rapides, Quebec, CanadaH7N 4Z3.
FAU - Dea, Serge
AU  - Dea S
AD  - Centre de Recherche en Sante Humaine1 et Centre de Recherche en Microbiologie et 
      Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, 
      Laval-des-Rapides, Quebec, CanadaH7N 4Z3.
FAU - St-Pierre, Yves
AU  - St-Pierre Y
AD  - Centre de Recherche en Sante Humaine1 et Centre de Recherche en Microbiologie et 
      Biotechnologie2, INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, 
      Laval-des-Rapides, Quebec, CanadaH7N 4Z3.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (RNA, Viral)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage
MH  - Collagen/metabolism
MH  - Enzyme Induction
MH  - Genome, Viral
MH  - Inflammation/enzymology/metabolism/pathology/virology
MH  - Lung/*enzymology/metabolism/pathology/virology
MH  - Matrix Metalloproteinases/blood/*metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*enzymology/metabolism/pathology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/isolation & 
      purification/*physiology
MH  - RNA, Viral/analysis
MH  - Spleen/virology
MH  - Swine/*virology
EDAT- 2001/05/23 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/05/23 10:00
PHST- 2001/05/23 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/05/23 10:00 [entrez]
AID - 10.1099/0022-1317-82-6-1253 [doi]
PST - ppublish
SO  - J Gen Virol. 2001 Jun;82(Pt 6):1253-1261. doi: 10.1099/0022-1317-82-6-1253.