PMID- 11370815
OWN - NLM
STAT- MEDLINE
DCOM- 20011011
LR  - 20061115
IS  - 0392-9078 (Print)
IS  - 0392-9078 (Linking)
VI  - 20
IP  - 1
DP  - 2001 Mar
TI  - A study of chromosome aneuploidy in hereditary breast cancer patients and their 
      healthy blood relatives.
PG  - 103-9
AB  - Chromosomal abnormalities that may predispose a group of individuals to develop 
      certain neoplasms have been reported in lymphocytes. We evaluated cytogenetic 
      abnormalities in 21 histopathologically confirmed primary breast cancer patients 
      (BCPs), 52 healthy blood relatives (HBRs), belonging to 19 hereditary breast 
      cancer families (HBFs) and 25 females as control. Phytohemagglutinin stimulated 
      peripheral blood lymphocyte (PBL) cultures were used to study the chromosomal 
      abnormalities in BCPs and their HBRs. Short term culture of the tumor tissue was 
      also carried out in defined growth medium. Suitable metaphases (11 to 55) from 
      tumors and a minimum of 100 metaphases from PBL were karyotyped for the 
      cytogenetic analysis. Heterogeneous population of cells with random and nonrandom 
      chromosomal abnormalities was noticed in tumors. In control groups 2-5% of 
      metaphases showed numerical abnormalities, whereas this phenomenon was observed 
      in 3-18% of metaphases in HBRs and 3-23% of metaphases in BCPs. In tumor tissue, 
      47.05% of BCPs showed numerical abnormalities in more than 16 metaphases. In 
      lymphocytes, this event was observed in 33.33% of BCPs and 13.14% of HBRs. In 
      controls 1.28%, in BCPs 52.04% (tumor) and 13.42% (lymphocytes), and in HBRs 
      9.03% of metaphases were found aneuploid. Statistically it was highly significant 
      (Fisher's exact test, P<0.00001). In lymphocytes of BCPs, chromosomes 1, 6, 8, 9, 
      15, 17, 18, 20, and X and in HBRs, chromosomes 8, 15, 17, 18, and X were 
      frequently involved. It can be inferred from the findings that the above 
      mentioned chromosomes may have an important role in early stage of breast 
      carcinogenesis in BCFs. Moreover, presence of similar abnormalities in HBR 
      indicates inherited pattern of this genetic error among them.
FAU - Roy, S K
AU  - Roy SK
AD  - Dept. of Surgical Oncology, The Gujarat Cancer Society, Asarwa, Ahmedabad, India. 
      shambhu@mail.ucr.edu
FAU - Trividi, A H
AU  - Trividi AH
FAU - Bakshi, S R
AU  - Bakshi SR
FAU - Patel, S J
AU  - Patel SJ
FAU - Shukla, P S
AU  - Shukla PS
FAU - Shah, A D
AU  - Shah AD
FAU - Majithiya, D B
AU  - Majithiya DB
FAU - Patel, D D
AU  - Patel DD
FAU - Shah, P M
AU  - Shah PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Clin Cancer Res
JT  - Journal of experimental & clinical cancer research : CR
JID - 8308647
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Breast Neoplasms/*genetics/immunology/pathology
MH  - Chromosomes, Human, Pair 13
MH  - Chromosomes, Human, Pair 17
MH  - Family
MH  - Female
MH  - Humans
MH  - Lymphocyte Activation
MH  - Lymphocytes/cytology/immunology/pathology
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Odds Ratio
MH  - Postmenopause
MH  - Premenopause
MH  - Reference Values
MH  - Trisomy
MH  - X Chromosome
EDAT- 2001/05/24 10:00
MHDA- 2001/10/12 10:01
CRDT- 2001/05/24 10:00
PHST- 2001/05/24 10:00 [pubmed]
PHST- 2001/10/12 10:01 [medline]
PHST- 2001/05/24 10:00 [entrez]
PST - ppublish
SO  - J Exp Clin Cancer Res. 2001 Mar;20(1):103-9.