PMID- 11372738
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20191025
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 73
IP  - 2
DP  - 2001 Feb
TI  - A Hodgkin's disease cell line, KM-H2, shows biphenotypic features of dendritic 
      cells and B cells.
PG  - 236-44
AB  - The origin of Reed-Sternberg (RS) cells, the neoplastic cells of Hodgkin's 
      disease, has long remained controversial. Dendritic cells (DCs) are highly 
      specialized antigen-presenting cells that have the unique capacity to prime naive 
      T cells, and they may be progenitors of RS cells in a population of Hodgkin's 
      disease cells. In this study, the KM-H2 cell line, previously established from a 
      patient with Hodgkin's disease of mixed cellularity, was reevaluated for its 
      cellular derivation, particularly in terms of DCs. KM-H2 cells were demonstrated 
      to carry the newly proposed DC-associated molecules fascin, CD83, and DEC-205, as 
      well as costimulatory molecules such as CD40, CD80, and CD86. In addition, KM-H2 
      cells were shown to be able to potently stimulate peripheral blood T cells and to 
      have the strong endocytotic activity of fluorescein isothiocyanate-dextran. On 
      the other hand, KM-H2 cells were shown to have variable-diversity-joining 
      recombination of the immunoglobulin H gene, although they did not express any 
      subclasses of immunoglobulin and they were negative for CD79a and CD79b. In 
      addition, KM-H2 cells produced the messenger RNA of the Pax-5 gene. These 
      findings lead to a hypothesis that KM-H2 cells originated from the cells that had 
      differentiated through the possible common DC-B-cell progenitors along the newly 
      proposed pathway.
FAU - Uehira, K
AU  - Uehira K
AD  - First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
FAU - Amakawa, R
AU  - Amakawa R
FAU - Ito, T
AU  - Ito T
FAU - Uehira, T
AU  - Uehira T
FAU - Ozaki, Y
AU  - Ozaki Y
FAU - Shimizu, T
AU  - Shimizu T
FAU - Fujimoto, M
AU  - Fujimoto M
FAU - Inaba, M
AU  - Inaba M
FAU - Fukuhara, S
AU  - Fukuhara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (Antigens, CD)
RN  - 0 (Carrier Proteins)
RN  - 0 (DEC-205 receptor)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (PAX5 Transcription Factor)
RN  - 0 (PAX5 protein, human)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Transcription Factors)
RN  - 146808-54-0 (fascin)
SB  - IM
MH  - Adult
MH  - *Antigens, CD
MH  - B-Lymphocytes/*pathology
MH  - Carrier Proteins/metabolism
MH  - Cell Lineage
MH  - DNA-Binding Proteins/metabolism
MH  - Dendritic Cells/*pathology
MH  - Flow Cytometry
MH  - Hodgkin Disease/*pathology
MH  - Humans
MH  - Immunophenotyping
MH  - *Lectins, C-Type
MH  - Lymphocyte Culture Test, Mixed
MH  - Male
MH  - Membrane Glycoproteins/metabolism
MH  - Microfilament Proteins/metabolism
MH  - Minor Histocompatibility Antigens
MH  - PAX5 Transcription Factor
MH  - Receptors, Cell Surface/metabolism
MH  - Reed-Sternberg Cells/pathology
MH  - Transcription Factors/metabolism
MH  - Tumor Cells, Cultured/chemistry/*pathology
EDAT- 2001/05/25 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/05/25 10:00
PHST- 2001/05/25 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/05/25 10:00 [entrez]
AID - 10.1007/BF02981944 [doi]
PST - ppublish
SO  - Int J Hematol. 2001 Feb;73(2):236-44. doi: 10.1007/BF02981944.