PMID- 11373057
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20181130
IS  - 0169-4197 (Print)
IS  - 0169-4197 (Linking)
VI  - 28
IP  - 3
DP  - 2000 Dec
TI  - Transforming growth factor alpha (TGF-alpha) increases cell number in a human 
      pancreatic cancer cell line but not in normal mouse pancreas.
PG  - 199-205
AB  - BACKGROUND: The pancreas harbors growth factors such as the epidermal growth 
      factor (EGF) family. The physiological and pathophysiological roles of growth 
      factors in normal pancreas remain unsettled. Human pancreatic cancer 
      overexpresses the EGF receptor, and the ligands EGF and transforming growth 
      factor alpha (TGF-alpha). The aim of the present experiments was to study the 
      effect of TGF-alpha in a pancreatic cancer cell line and in normal mouse 
      pancreas. METHOD: The LN-36 cell line, established from a pancreatic duct cell 
      adenocarcinoma, was incubated with TGF-alpha or EGF. The effect of an EGF 
      receptor-specific, tyrosine kinase inhibitor (tyrphostin B56) with or without 
      growth factors was also studied. The cell number was measured with the 
      XTT-colorimetric method. TGF-alpha, the tyrphostins A25, B48, and B56, were in 
      separate experiments infused during 1 wk to normal female mice by subcutaneous 
      (sc) minipumps. RESULTS: The LN-36 cell line responded to TGF-alpha and EGF with 
      increased cell number; +61% with 10(-10) M TGF-alpha and +34% with 10(-9) M EGF. 
      Tyrphostin B56 at a concentration of 10(-5) M reduced the cell number by 76%, but 
      when incubated together with growth factors the reduction was only 44% with 
      TGF-alpha, and 39% with EGF. Infusion of TGF-alpha increased mouse pancreatic wet 
      weight and protein content but was without effect on DNA synthesis, measured as 
      incorporation of tritiated thymidine. Infusion of three different tyrphostins did 
      not influence mice pancreas. CONCLUSION: The results support the role of 
      TGF-alpha to maintain growth of pancreatic cancer cells by the EGF receptor. 
      Infusion of TGF-alpha induced hypertrophy in normal mouse pancreas.
FAU - Kullenberg, B
AU  - Kullenberg B
AD  - Department of Surgery, Lund University, Sweden.
FAU - Jansen, C
AU  - Jansen C
FAU - Fredang, N
AU  - Fredang N
FAU - Ohlsson, B
AU  - Ohlsson B
FAU - Axelson, J
AU  - Axelson J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Pancreatol
JT  - International journal of pancreatology : official journal of the International 
      Association of Pancreatology
JID - 8703511
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (Tyrphostins)
RN  - 149092-35-3 (AG 556)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Dose-Response Relationship, Drug
MH  - Epidermal Growth Factor/pharmacology
MH  - ErbB Receptors/physiology
MH  - Female
MH  - Humans
MH  - Hypertrophy
MH  - Mice
MH  - Pancreas/*drug effects/pathology
MH  - Pancreatic Neoplasms/*pathology
MH  - Transforming Growth Factor alpha/*pharmacology
MH  - Tumor Cells, Cultured
MH  - Tyrphostins/pharmacology
EDAT- 2001/05/25 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/05/25 10:00
PHST- 2001/05/25 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/05/25 10:00 [entrez]
AID - 10.1385/IJGC:28:3:199 [doi]
PST - ppublish
SO  - Int J Pancreatol. 2000 Dec;28(3):199-205. doi: 10.1385/IJGC:28:3:199.