PMID- 11374044
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20191104
IS  - 1435-2443 (Print)
IS  - 1435-2443 (Linking)
VI  - 386
IP  - 2
DP  - 2001 Mar
TI  - Quantification of alpha 1-fetoprotein mRNA in peripheral blood and bone marrow: a 
      tool for perioperative evaluation of patients with hepatocellular carcinoma.
PG  - 118-23
AB  - BACKGROUND/AIMS: Quantification of alpha 1-fetoprotein (AFP) mRNA in the blood 
      using reverse transcriptase polymerase chain reaction (RT-PCR) could be a useful 
      tool in monitoring the dynamics of minimal residual disease in patients with 
      hepatocellular carcinoma (HCC). Since all available assays do not take into 
      account the efficiency of cell separation, RNA extraction and reverse 
      transcription, a competitive RT-PCR assay for quantification of AFP mRNA in 
      relation to the housekeeping gene glyceraldehyde phosphate dehydrogenase (GAPDH) 
      was established. PATIENTS AND METHODS: Peripheral blood of 22 patients and bone 
      marrow aspirates of 11 patients with hepatocellular carcinoma was monitored 
      perioperatively. Eighteen patients with other hepatic tumours or non-malignant 
      hepatic diseases and 26 healthy blood donors served as controls. Messenger RNA 
      contents were calculated relative to the content of GAPDH mRNA as an indicator of 
      total cell count. RESULTS: Among HCC patients, 6 of 22 (26%) were positive for 
      AFP mRNA before operation with values ranging from 2 ag/100 fg to 36 ag/100 fg 
      GAPDH mRNA (mean 14). Among bone marrow samples, AFP mRNA was detectable in 5 of 
      11 (45%) cases, with 4 ag/100 fg to 23 ag/100 fg GAPDH (mean 9). However, AFP 
      mRNA was also detectable in 3 of 18 (17%) control patients and in 2 of 26 (8%) 
      healthy blood donors. Perioperative findings were highly variable. CONCLUSION: 
      AFP mRNA is not a specific marker for circulating malignant hepatocytes. Whether 
      definition of a cut-off level or the use of a multimarker-PCR will provide more 
      useful data remains to be established.
FAU - Aselmann, H
AU  - Aselmann H
AD  - Klinik fur Viszeral- und Transplantationschirurgie, Medizinische Hochschule 
      Hannover, Carl-Neuberg-Strasse 1, 30623 Hannover, Germany.
FAU - Wolfes, H
AU  - Wolfes H
FAU - Rohde, F
AU  - Rohde F
FAU - Frerker, M
AU  - Frerker M
FAU - Deiwick, A
AU  - Deiwick A
FAU - Jager, M D
AU  - Jager MD
FAU - Klempnauer, J
AU  - Klempnauer J
FAU - Schlitt, H J
AU  - Schlitt HJ
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Langenbecks Arch Surg
JT  - Langenbeck's archives of surgery
JID - 9808285
RN  - 0 (RNA, Messenger)
RN  - 0 (alpha-Fetoproteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow/metabolism
MH  - Carcinoma, Hepatocellular/blood/*metabolism
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Liver Neoplasms/blood/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Neoplastic Cells, Circulating/metabolism
MH  - Polymerase Chain Reaction
MH  - Predictive Value of Tests
MH  - Preoperative Care
MH  - RNA, Messenger/blood/*metabolism
MH  - alpha-Fetoproteins/*metabolism
EDAT- 2001/05/26 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/05/26 10:00
PHST- 2001/05/26 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/05/26 10:00 [entrez]
AID - 10.1007/s004230000199 [doi]
PST - ppublish
SO  - Langenbecks Arch Surg. 2001 Mar;386(2):118-23. doi: 10.1007/s004230000199.