PMID- 11374886
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 284
IP  - 1
DP  - 2001 Jun 1
TI  - Inhibition of Th1- and enhancement of Th2-initiating cytokines and chemokines in 
      trichosanthin- treated macrophages.
PG  - 168-72
AB  - Trichosanthin (TCS), the major effective component from Chinese herb 
      Trichosanthes Kirilowii Maxim, is also a potent allergen. Our previous work has 
      shown that TCS can upregulate interleukin-4 (IL-4) and interleukin-13 (IL-13) 
      while inhibit interferon-gamma (IFN-gamma) in mesenteric lymph node cells after 
      TCS immunization. Thus, TCS can arouse a T helper 2 (Th2) response in the 
      draining lymph node. However, little is known about the early effects of TCS on 
      antigen-presenting cells, the initiator of T cell response. In the current study, 
      the effects of TCS on macrophage cytokines and chemokine expression were 
      investigated. Peritoneal macrophages were treated with or without TCS in the 
      presence of lipopolysaccharide (LPS). We found that TCS increased macrophage 
      interleukin-10 (IL-10) and monocyte chemoattractant protein-1 (MCP-1) expression, 
      whereas it decreased interleukin-12 (IL-12) and tumor necrosis factor-alpha 
      (TNF-alpha) expression. Our study clearly demonstrated that TCS, as an allergen, 
      has differential effects on macrophage Th1/Th2 initiative factors, effects that 
      are likely to facilitate its inducing of Th2 and immunoglobulin E (IgE) response.
CI  - Copyright 2001 Academic Press.
FAU - Xu, W
AU  - Xu W
AD  - Laboratory of Molecular Immunology, Institute of Biochemistry and Cell Biology, 
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 
      Shanghai, 200031, China.
FAU - Hou, W
AU  - Hou W
FAU - Yao, G
AU  - Yao G
FAU - Ji, Y
AU  - Ji Y
FAU - Yeh, M
AU  - Yeh M
FAU - Sun, B
AU  - Sun B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Allergens)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 60318-52-7 (Trichosanthin)
SB  - IM
MH  - Allergens/immunology/pharmacology
MH  - Animals
MH  - Cell Count
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Chemokines/genetics/*metabolism/pharmacology
MH  - Cytokines/genetics/*metabolism/pharmacology
MH  - Female
MH  - Interleukin-10/genetics/metabolism
MH  - Interleukin-12/genetics/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophage Activation/drug effects
MH  - Macrophages, Peritoneal/cytology/drug effects/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
MH  - Trichosanthin/immunology/*pharmacology
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
EDAT- 2001/05/26 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/05/26 10:00
PHST- 2001/05/26 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/05/26 10:00 [entrez]
AID - S0006-291X(01)94940-X [pii]
AID - 10.1006/bbrc.2001.4940 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Jun 1;284(1):168-72. doi: 
      10.1006/bbrc.2001.4940.