PMID- 11376053
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 39
IP  - 6
DP  - 2001 Jun
TI  - Frequent detection of the human herpesvirus 6-specific genomes in the livers of 
      children with various liver diseases.
PG  - 2173-7
AB  - This study was performed to investigate the frequency of human herpesvirus 6 
      (HHV-6) infection of the liver in children with a variety of liver diseases and 
      to evaluate the role of HHV-6 infection in pediatric patients with prolonged 
      non-B non-C hepatitis. Detection of the HHV-6 genomes in liver, in peripheral 
      blood mononuclear cells (PBMC), and in plasma was performed by PCR or by in situ 
      hybridization. Liver biopsy materials from 48 patients, in whom HHV-6 infection 
      was serologically confirmed, were available for PCR analysis. Sequences of the 
      HHV-6B genome were detectable in the livers of 36 of 48 patients (75%). The 
      presence of the genome was not associated with serum transaminase activities. The 
      genome was detectable in PBMC of 22 of 31 (71%) patients tested. In these 31 
      patients HHV-6 was detected in both the livers and PBMC of 20, was detected in 
      PBMC but not in the livers of 2, was detected in the livers but not in PBMC of 3, 
      and was detected in neither of samples of 6. In situ hybridization of the livers 
      of six patients showed the presence of the HHV-6B genome in the nuclei of 
      hepatocytes. The anti-HHV-6 immunoglobulin M antibody was detectable in 2 of 9 of 
      the non-B non-C hepatitis patients, whereas none of the 22 patients with 
      etiology-defined liver diseases tested positive. Cell-free viral DNA was not 
      detectable in either group of patients. Our results showed that HHV-6B is 
      frequently present in the livers of children with a variety of liver diseases but 
      do not support the assumption that HHV-6B infection of the liver is associated 
      with prolonged non-B non-C hepatitis.
FAU - Ozaki, Y
AU  - Ozaki Y
AD  - Department of Pediatrics, Osaka University Graduate School of Medicine, 2-2 
      Yamadaoka Suita-shi, Osaka 565-0871, Japan.
FAU - Tajiri, H
AU  - Tajiri H
FAU - Tanaka-Taya, K
AU  - Tanaka-Taya K
FAU - Mushiake, S
AU  - Mushiake S
FAU - Kimoto, A
AU  - Kimoto A
FAU - Yamanishi, K
AU  - Yamanishi K
FAU - Okada, S
AU  - Okada S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Viral/blood
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/analysis/blood
MH  - Female
MH  - *Genome, Viral
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 6, Human/genetics/immunology/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization
MH  - Infant
MH  - Liver/*virology
MH  - Liver Diseases/*virology
MH  - Male
MH  - Polymerase Chain Reaction/methods
PMC - PMC88107
EDAT- 2001/05/29 10:00
MHDA- 2001/08/24 10:01
PMCR- 2001/06/01
CRDT- 2001/05/29 10:00
PHST- 2001/05/29 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/05/29 10:00 [entrez]
PHST- 2001/06/01 00:00 [pmc-release]
AID - 1208 [pii]
AID - 10.1128/JCM.39.6.2173-2177.2001 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2001 Jun;39(6):2173-7. doi: 10.1128/JCM.39.6.2173-2177.2001.