PMID- 11376078 OWN - NLM STAT- MEDLINE DCOM- 20010823 LR - 20191210 IS - 0095-1137 (Print) IS - 1098-660X (Electronic) IS - 0095-1137 (Linking) VI - 39 IP - 6 DP - 2001 Jun TI - Development of reverse transcriptase PCR assays for detection of active human herpesvirus 6 infection. PG - 2308-10 AB - We developed reverse transcriptase (RT) PCR assays for the detection of mRNA from three spliced genes of human herpesvirus 6 (HHV-6), the immediate-early genes U16/U17 and U89/U90 and the late gene U60/U66. Sequence analysis determined the splicing sites of these genes. The new assays may be instrumental in investigating the association between HHV-6 and disease. FAU - Van den Bosch, G AU - Van den Bosch G AD - Laboratory of Experimental Hematology, University of Antwerp, Antwerp University Hospital, Wilrijkstraat 10, B-2650 Edegem, Belgium. FAU - Locatelli, G AU - Locatelli G FAU - Geerts, L AU - Geerts L FAU - Faga, G AU - Faga G FAU - Ieven, M AU - Ieven M FAU - Goossens, H AU - Goossens H FAU - Bottiger, D AU - Bottiger D FAU - Oberg, B AU - Oberg B FAU - Lusso, P AU - Lusso P FAU - Berneman, Z N AU - Berneman ZN LA - eng SI - GENBANK/AF241835 SI - GENBANK/AF241836 SI - GENBANK/AF241837 SI - GENBANK/AF241838 SI - GENBANK/AF241839 SI - GENBANK/AF241840 PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Clin Microbiol JT - Journal of clinical microbiology JID - 7505564 RN - 0 (Immediate-Early Proteins) RN - 0 (RNA, Messenger) RN - 0 (Viral Proteins) SB - IM MH - Genes, Immediate-Early MH - Genes, Viral MH - Herpesviridae Infections/*diagnosis/virology MH - Herpesvirus 6, Human/genetics/*isolation & purification MH - Humans MH - Immediate-Early Proteins/genetics MH - Molecular Sequence Data MH - RNA Splicing MH - RNA, Messenger/metabolism MH - *Reverse Transcriptase Polymerase Chain Reaction MH - Sequence Analysis, DNA MH - Viral Proteins/genetics PMC - PMC88132 EDAT- 2001/05/29 10:00 MHDA- 2001/08/24 10:01 PMCR- 2001/06/01 CRDT- 2001/05/29 10:00 PHST- 2001/05/29 10:00 [pubmed] PHST- 2001/08/24 10:01 [medline] PHST- 2001/05/29 10:00 [entrez] PHST- 2001/06/01 00:00 [pmc-release] AID - 1174 [pii] AID - 10.1128/JCM.39.6.2308-2310.2001 [doi] PST - ppublish SO - J Clin Microbiol. 2001 Jun;39(6):2308-10. doi: 10.1128/JCM.39.6.2308-2310.2001.