PMID- 11376552 OWN - NLM STAT- MEDLINE DCOM- 20011025 LR - 20191104 IS - 1341-7568 (Print) IS - 1341-7568 (Linking) VI - 76 IP - 1 DP - 2001 Feb TI - Identification and chromosomal location of tandemly repeated DNA sequences in Allium cepa. PG - 53-60 AB - A 314-bp tandemly repeated DNA sequence, named pAc074, was characterized in Allium cepa by fluorescence in situ hybridization (FISH) analyses using random amplified fragment as probe. The nucleotide sequences of the clone pAc074 is partially homologous to the satellite DNA sequences, ACSAT1, ACSAT2, and ACSAT3, of A. cepa with 81%, 81% and 78% similarity, respectively. Our sequential C-banding and FISH with pAc074 probe also clearly showed a close relation between Cheterochromatin at telomeric region and pAc074 sequences on all the chromosomes except on chromosome 6. On the long arm of chromosome 7, pAc074 sequences appeared as interstitial band which did not correspond to C-heterochromatin bands. Instead, the C-heterochromatin bands corresponded with the 5S rDNA signals. This is the first evidence of simultaneous banding of the 5S rDNA and C-band in A. cepa. FAU - Do, G S AU - Do GS AD - Department of Biology, Kyungpook National University, Taegu, Korea. FAU - Seo, B B AU - Seo BB FAU - Yamamoto, M AU - Yamamoto M FAU - Suzuki, G AU - Suzuki G FAU - Mukai, Y AU - Mukai Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Japan TA - Genes Genet Syst JT - Genes & genetic systems JID - 9607822 RN - 0 (DNA Primers) RN - 0 (DNA, Plant) RN - 0 (Heterochromatin) SB - IM MH - Base Sequence MH - Chromosome Banding MH - Chromosome Mapping MH - DNA Primers/genetics MH - DNA, Plant/genetics MH - Heterochromatin/genetics MH - In Situ Hybridization, Fluorescence MH - Molecular Sequence Data MH - Onions/*genetics MH - Polymerase Chain Reaction MH - Sequence Homology, Nucleic Acid MH - Tandem Repeat Sequences EDAT- 2001/05/30 10:00 MHDA- 2001/10/26 10:01 CRDT- 2001/05/30 10:00 PHST- 2001/05/30 10:00 [pubmed] PHST- 2001/10/26 10:01 [medline] PHST- 2001/05/30 10:00 [entrez] AID - 10.1266/ggs.76.53 [doi] PST - ppublish SO - Genes Genet Syst. 2001 Feb;76(1):53-60. doi: 10.1266/ggs.76.53.