PMID- 11376844
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20190910
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 76
IP  - 1
DP  - 2001 Jul
TI  - Prevalence of genotypes 1 and 2 of bovine viral diarrhea virus in Lower Saxony, 
      Germany.
PG  - 31-42
AB  - The aim of this study was to find whether an antigenic drift had occurred in 
      Lower Saxony in the past 40 years. For this, the genetic diversity of bovine 
      viral diarrhea virus (BVDV) isolates mainly from Lower Saxony was estimated by 
      RT-PCR and sequencing of a 420 bp fragment of the E2 glycoprotein gene. Sixty-one 
      field virus isolates collected during routine diagnostics between 1960 and 2000 
      in Lower Saxony, Northern Germany, were analyzed. Phylogenetic analysis allowed 
      discrimination of genotypes BVDV 1 and 2. Excepting two isolates, which were of 
      BVDV type 2, most of the isolates were classified as BVDV type 1. This group 
      could be further subdivided into four subgroups and one disparate isolate. 
      Independent of the year of isolation and geographical localization, 54 isolates 
      clustered in two subtypes (BVDV subtypes 1b and 1d). Only one isolate was 
      classified as BVDV type 1a, thus being similar to the North American NADL strain, 
      and to the vaccine strain Oregon C24V, which was extensively used for vaccination 
      in Germany. The remaining isolates belonged to new clusters tentatively 
      designated as BVDV subtypes 1g and 1f. To compare the cluster designation with 
      that of other studies, phylogenetic analysis of representatives of each of the 
      subgroups based on the 5' untranslated region (5'UTR) was performed. It grouped 
      the viruses similarly. The results indicate that the BVDV population seems to be 
      relatively stable over 40 years in Lower Saxony.
FAU - Tajima, M
AU  - Tajima M
AD  - Department of Clinical Sciences, School of Veterinary Medicine, Hokkaido 
      University, 060-0818, Sapporo, Japan.
FAU - Frey, H R
AU  - Frey HR
FAU - Yamato, O
AU  - Yamato O
FAU - Maede, Y
AU  - Maede Y
FAU - Moennig, V
AU  - Moennig V
FAU - Scholz, H
AU  - Scholz H
FAU - Greiser-Wilke, I
AU  - Greiser-Wilke I
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Animals
MH  - Bovine Virus Diarrhea-Mucosal Disease/epidemiology/*virology
MH  - Cattle
MH  - Diarrhea Virus 1, Bovine Viral/*classification/*genetics/isolation & purification
MH  - Diarrhea Virus 2, Bovine Viral/*classification/*genetics/isolation & purification
MH  - Gene Frequency/genetics
MH  - Genotype
MH  - Germany
MH  - Phylogeny
MH  - Prevalence
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Viral Envelope Proteins/genetics
EDAT- 2001/05/30 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/05/30 10:00
PHST- 2001/05/30 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/05/30 10:00 [entrez]
AID - S0168-1702(01)00244-1 [pii]
AID - 10.1016/s0168-1702(01)00244-1 [doi]
PST - ppublish
SO  - Virus Res. 2001 Jul;76(1):31-42. doi: 10.1016/s0168-1702(01)00244-1.