PMID- 11376957
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20190901
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 81
IP  - 2
DP  - 2001 Jul 26
TI  - Semen from boars infected with porcine reproductive and respiratory syndrome 
      virus (PRRSV) contains antibodies against structural as well as nonstructural 
      viral proteins.
PG  - 109-25
AB  - The seminal excretion of antibodies against porcine reproductive and respiratory 
      syndrome virus (PRRSV) was examined in a group of five boars experimentally 
      infected by the nasopharyngeal route. By using phage-displayed peptide epitopes 
      from the PRRSV replicase and envelope glycoproteins as ELISA antigen, we were 
      able to separately and specifically assay antibody responses against structural 
      and nonstructural viral proteins. Antibodies against structural as well as 
      nonstructural viral proteins were consistently found in the semen of all boars, 
      beginning from 1-4 weeks postinfection. This is the first report documenting the 
      presence of anti-PRRSV antibodies in boar semen. Seminal antiviral IgA was also 
      detected, and we observed a correlation between seminal IgA responses against 
      nonstructural viral proteins, and the duration of PRRSV RNA excretion in semen. 
      The implications of these findings for the diagnostics and pathogenesis of 
      venereal PRRSV infection are discussed.
FAU - Oleksiewicz, M B
AU  - Oleksiewicz MB
AD  - Danish Veterinary Institute for Virus Research, Lindholm, 4771 Kalvehave, 
      Denmark. mo@vetvirus.dk
FAU - Botner, A
AU  - Botner A
FAU - Normann, P
AU  - Normann P
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin A)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Viral/*analysis/blood
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Epitopes
MH  - Immunoglobulin A/analysis/blood
MH  - Male
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - RNA, Viral/chemistry/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Semen/*immunology/virology
MH  - Swine
MH  - Time Factors
MH  - Viral Proteins/*immunology
EDAT- 2001/05/30 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/05/30 10:00
PHST- 2001/05/30 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/05/30 10:00 [entrez]
AID - S0378-1135(01)00341-8 [pii]
AID - 10.1016/s0378-1135(01)00341-8 [doi]
PST - ppublish
SO  - Vet Microbiol. 2001 Jul 26;81(2):109-25. doi: 10.1016/s0378-1135(01)00341-8.