PMID- 11378326
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20220316
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 26
IP  - 2
DP  - 2001 Jun
TI  - Role of stem cell factor and monocyte chemoattractant protein-1 in the 
      interaction between fibroblasts and mast cells in fibrosis.
PG  - 106-11
AB  - Mast cell infiltration and accumulation is known to occur in tissue fibrosis. 
      Increased numbers of mast cells are detected in scleroderma or hypertrophic scar 
      skin, however, neither the role of mast cells nor the interaction of fibroblasts 
      and mast cells in fibrosis are fully understood. A growing body of evidence 
      indicate that mast cells are rich source of cytokines, growth factors or 
      chemokines, which are suggested to play an important role in the induction of 
      fibrosis. Recent in vivo and in vitro studies suggest the involvement of monocyte 
      chemoattractant protein-1 (MCP-1), a member of the C-C chemokine family, in 
      fibrosis. Here, we examined the effect of stem cell factor (SCF), a mast cell 
      growth factor, on MCP-1 gene expression in a human mast cell line, HMC-1, and as 
      well as the effect of MCP-1 on alpha1(I) collagen gene expression in human skin 
      fibroblasts. HMC-1 cells spontaneously expressed MCP-1 mRNA transcripts, which 
      was detectable by in situ hybridization and Northern blot analysis. Stimulation 
      with SCF further upregulated MCP-1 mRNA expression in a time- and dose-dependent 
      manner, and stimulation with 100 ng/ml SCF for 24 h induced a 3-fold increase of 
      MCP-1 mRNA expression in HMC-1 cells as compared with unstimulated cells. The 
      concentration of MCP-1 protein in the culture supernatants of 50 ng/ml 
      SCF-stimulated HMC-1 cells (3816+/-70 pg/ml) was significantly elevated compared 
      to unstimulated cells (2588+/-130 pg/ml) (P < 0.01), as assessed by ELISA. 
      Adversely, MCP-1 induced alpha1(I) collagen mRNA expression in normal skin 
      fibroblasts dose-dependently. Finally, comparative study revealed that the 
      concentration of SCF in the culture supernatants of scleroderma fibroblasts at 
      primary passages was significantly increased (344.6+/-182.4 pg/ml), as compared 
      with normal skin fibroblasts (72.4+/-20.2 pg/ml) (P<0.05). These results suggest 
      that fibroblast-derived SCF upregulates MCP-1 expression and synthesis in mast 
      cells, which acts on fibroblasts to enhance alpha1(I) collagen mRNA expression. 
      Our data may indicate an important interaction of fibroblasts and mast cells, via 
      SCF and MCP-1, in the induction of fibrosis.
FAU - Yamamoto, T
AU  - Yamamoto T
AD  - Department of Dermatology, University of Cologne, Cologne, Germany.
FAU - Hartmann, K
AU  - Hartmann K
FAU - Eckes, B
AU  - Eckes B
FAU - Krieg, T
AU  - Krieg T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Stem Cell Factor)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Cell Communication
MH  - Cell Line
MH  - Chemokine CCL2/genetics/*physiology
MH  - Collagen/genetics
MH  - Fibroblasts/drug effects/pathology/*physiology
MH  - Fibrosis
MH  - Humans
MH  - Mast Cells/drug effects/pathology/*physiology
MH  - RNA, Messenger/genetics/metabolism
MH  - Skin/pathology/physiopathology
MH  - Stem Cell Factor/*pharmacology
MH  - Up-Regulation/drug effects
EDAT- 2001/05/30 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/05/30 10:00
PHST- 2001/05/30 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/05/30 10:00 [entrez]
AID - S0923-1811(00)00164-X [pii]
AID - 10.1016/s0923-1811(00)00164-x [doi]
PST - ppublish
SO  - J Dermatol Sci. 2001 Jun;26(2):106-11. doi: 10.1016/s0923-1811(00)00164-x.