PMID- 11378330
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20191025
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 26
IP  - 2
DP  - 2001 Jun
TI  - Expression of monocyte chemoattractant protein-1 in the lesional skin of systemic 
      sclerosis.
PG  - 133-9
AB  - Systemic sclerosis (SSc) is a connective tissue disease with unknown etiology 
      characterized by excessive deposition of collagen in the skin as well as various 
      internal organs. One of the characteristic histological features is the presence 
      of infiltrating mononuclear cells in the dermis in its early stage. As well as T 
      cells, macrophages are implicated to play an important role in the initial 
      pathologic changes associated with SSc by releasing fibrogenic cytokines, 
      including transforming growth factor-beta or platelet-derived growth factor. 
      However, the precise mechanism for increased monocyte/macrophage recruitment in 
      the lesional skin of SSc is still not completely elucidated. Monocyte 
      chemoattractant protein-1 (MCP-1) is a predominant monocyte chemoattractant 
      secreted by various cells types including mononuclear cells, fibroblasts, smooth 
      muscle cells, endothelial cells, or keratinocytes. In this study, we examined the 
      expression of MCP-1 protein and mRNA in the lesional skin of seven patients with 
      SSc by immunohistochemistry and in situ hybridization. Results of 
      immunohistochemistry showed that MCP-1 was detected on infiltrating mononuclear 
      cells and fibroblastic cells in scleroderma skin, whereas normal skin showed only 
      minimal MCP-1 expression. We demonstrated the expression of MCP-1 mRNA in 
      infiltrating mononuclear cells and keratinocytes in scleroderma and contact 
      dermatitis skin. In addition, signals were also detected in fibroblasts in the 
      lesional skin of scleroderma, whereas fibroblasts in normal skin and contact 
      dermatitis skin did not express MCP-1 mRNA. These findings suggest that MCP-1 
      plays a role in recruiting monocyte/macrophages in the lesional skin of 
      scleroderma and that activated fibroblasts in scleroderma are involved in this 
      process.
FAU - Yamamoto, T
AU  - Yamamoto T
AD  - Department of Dermatology, University of Cologne, Joseph-Stelzmann Street 9, 
      50924, Cologne, Germany. yamamoto.derm@med.tmd.ac.jp
FAU - Eckes, B
AU  - Eckes B
FAU - Hartmann, K
AU  - Hartmann K
FAU - Krieg, T
AU  - Krieg T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Chemokine CCL2/*genetics/*metabolism
MH  - Female
MH  - Fibroblasts/metabolism/pathology
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - Macrophages/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/metabolism/pathology
MH  - RNA, Messenger/genetics/metabolism
MH  - Scleroderma, Systemic/*metabolism/pathology
MH  - Skin/*metabolism/pathology
EDAT- 2001/05/30 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/05/30 10:00
PHST- 2001/05/30 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/05/30 10:00 [entrez]
AID - S0923-1811(00)00169-9 [pii]
AID - 10.1016/s0923-1811(00)00169-9 [doi]
PST - ppublish
SO  - J Dermatol Sci. 2001 Jun;26(2):133-9. doi: 10.1016/s0923-1811(00)00169-9.