PMID- 11378391
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20190728
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 11
IP  - 10
DP  - 2001 May 15
TI  - Transcriptional repression by suppressor of hairless involves the binding of a
      hairless-dCtBP complex in Drosophila.
PG  - 789-92
AB  - Notch is the receptor for a conserved signaling pathway that regulates numerous
      cell fate decisions during development [1]. Signal transduction involves the
      presenilin-dependent intracellular processing of Notch and the nuclear
      translocation of the intracellular domain of Notch, NICD [2-6]. NICD associates
      with Suppressor of Hairless [Su(H)], a DNA binding protein, and Mastermind (Mam),
      a transcriptional coactivator [7-9]. In the absence of Notch signaling, Su(H)
      acts as a transcriptional repressor [10, 11]. Repression by Su(H) is relieved by 
      the activation of Notch [12-16]. In the Drosophila embryo, this transcriptional
      switch from repression to activation is important for patterning the expression
      of the single-minded (sim) gene along the dorsoventral axis [12]. Here, we
      investigate the mechanisms by which Su(H) inhibits the expression of Notch target
      genes in Drosophila. We show that Hairless, an antagonist of Notch signaling
      [17-19], is required to repress the transcription of the sim gene. Hairless forms
      a DNA-bound complex with Su(H). Furthermore, it directly binds the Drosophila
      C-terminal Binding Protein (dCtBP), which acts as a transcriptional corepressor. 
      The dCtBP binding motif of Hairless is essential for the function of Hairless in 
      vivo. We propose that Hairless mediates transcriptional repression by Su(H) via
      the recruitment of dCtBP.
FAU - Morel, V
AU  - Morel V
AD  - Ecole Normale Superieure, Unite Mixte de Recherche 8544, 46 rue d'Ulm, 75230,
      Cedex 05, Paris, France.
FAU - Lecourtois, M
AU  - Lecourtois M
FAU - Massiani, O
AU  - Massiani O
FAU - Maier, D
AU  - Maier D
FAU - Preiss, A
AU  - Preiss A
FAU - Schweisguth, F
AU  - Schweisguth F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insect Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Su(H) protein, Drosophila)
RN  - 0 (Transcription Factors)
RN  - 148349-66-0 (H protein, Drosophila)
RN  - EC 1.1.- (Alcohol Oxidoreductases)
RN  - EC 1.1.1.- (C-terminal binding protein)
SB  - IM
MH  - Alcohol Oxidoreductases
MH  - Amino Acid Sequence
MH  - Animals
MH  - DNA-Binding Proteins/*metabolism
MH  - Drosophila/embryology
MH  - *Drosophila Proteins
MH  - Insect Proteins/*metabolism
MH  - Phosphoproteins/*metabolism
MH  - Protein Binding
MH  - Repressor Proteins/*physiology
MH  - Sequence Homology, Amino Acid
MH  - *Transcription Factors
MH  - *Transcription, Genetic
EDAT- 2001/05/30 10:00
MHDA- 2001/07/13 10:01
CRDT- 2001/05/30 10:00
PHST- 2001/05/30 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/05/30 10:00 [entrez]
AID - S0960-9822(01)00224-X [pii]
AID - 10.1016/s0960-9822(01)00224-x [doi]
PST - ppublish
SO  - Curr Biol. 2001 May 15;11(10):789-92. doi: 10.1016/s0960-9822(01)00224-x.