PMID- 11378868
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20191210
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 44
IP  - 2
DP  - 2001 Jun 1
TI  - Automated evaluation of frequencies of aneuploid sperm by laser-scanning 
      cytometry (LSC).
PG  - 156-60
AB  - BACKGROUND: Laser-scanning cytometry (LSC) allows fast automated scoring of 
      fluorescence signals directly on microscopic slides. Frequencies of spontaneous 
      aneuploidies in murine and human sperm were evaluated by using this new LSC 
      technique. Rapid detection may be of great interest in reproductive toxicology, 
      as certain chemicals act as aneugens during meiosis, increasing the production of 
      aneuploid germ cells. Materials and Methods Selected chromosomes were detected by 
      using fluorescence in situ hybridization (FISH) and fluorochrome-labeled 
      DNA-probes. Sperm chromatin was counterstained with propidium iodide. By scanning 
      across the slide, fluorescence signals within sperm nuclei were detected and 
      counted. RESULTS: In murine sperm, the frequencies of disomies for chromosomes 8 
      and X were 0.019% and 0.021%, respectively. The automated assessment in human 
      sperm resulted in disomy frequencies of 0.061% and 0.090% for chromosomes 13 and 
      X, respectively. These results were comparable to data obtained from the same 
      samples by manual microscopic scoring and to literature data. CONCLUSIONS: 
      Frequencies of genotypically abnormal sperm were not significantly different 
      between automated and manual scoring. In conclusion, sperm aneuploidy was 
      reliably determined and disomic sperm were successfully relocated by LSC. By 
      virtue of rapid and reliable analyses, LSC has the powerful potential to replace 
      manual microscopic FISH analysis in molecular cytogenetics.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Baumgartner, A
AU  - Baumgartner A
AD  - Institute of Experimental Genetics, GSF-National Research Center for Environment 
      and Health, Neuherberg, Germany. baumgartner@gsf.de
FAU - Schmid, T E
AU  - Schmid TE
FAU - Maerz, H K
AU  - Maerz HK
FAU - Adler, I D
AU  - Adler ID
FAU - Tarnok, A
AU  - Tarnok A
FAU - Nuesse, M
AU  - Nuesse M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - Automation
MH  - DNA/*analysis
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Lasers
MH  - Male
MH  - Mice
MH  - Mice, Inbred C3H
MH  - *Spermatozoa
EDAT- 2001/05/30 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/05/30 10:00
PHST- 2001/05/30 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/05/30 10:00 [entrez]
AID - 10.1002/1097-0320(20010601)44:2<156::AID-CYTO1096>3.0.CO;2-5 [pii]
PST - ppublish
SO  - Cytometry. 2001 Jun 1;44(2):156-60.