PMID- 11379084
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20161124
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 43
IP  - 1
DP  - 2001
TI  - Contribution of Pseudomonas spp. to phosphorus uptake in the anoxic zone of an 
      anaerobic-anoxic-aerobic continuous activated sludge system.
PG  - 139-46
AB  - A continuously operated laboratory-scale (32 L) nitrification denitrification 
      biological excess phosphorus removal (NDBEPR) activated sludge system (modeled on 
      the 3-stage Phoredox configuration) was maintained for 140 d. The transition from 
      a non-biological excess phosphorus removal (BEPR) sludge to one exhibiting a 
      strong BEPR mechanism was monitored. Mixed liquor seed inoculum was obtained from 
      a full-scale single aerobic activated sludge installation and subjected to 
      conditions conducive to BEPR, i.e. increasing influent acetate (HAc) 
      concentrations. At a sludge age of 10 d with 100% HAc feed, the system was 
      capable of removing a maximum of ca. 40 mgPO4-P/L from the bulk liquid; P/VSS of 
      ca. 0.27 (mgP/mgVSS); and VSS/TSS of 0.53 (mgVSS/mgTSS) in the aerobic zone was 
      attained. Although typical BEPR phosphorus transformation patterns were routinely 
      observed, i.e. anaerobic phosphate release and aerobic phosphate uptake, 
      phosphate uptake in the anoxic zone was also recorded indicating the presence of 
      denitrifying phosphorus accumulating organisms (DPAOs) in the sludge community. 
      The microbial community was screened (using both isolation and direct methods of 
      analysis) for the presence of Pseudomonas spp. as this genus is known to perform 
      both polyphosphate accumulation and denitrification processes. Isolation of 
      anoxic mixed liquor bacteria on solid media and identification using the APl 20NE 
      system resulted in the total dominance of the Pseudomonads (> 50%). However, 
      direct fluorescent in situ hybridizations (FISH) revealed that Pseudomonas spp. 
      only constituted ca. 3% of the total bacterial community indicating that other 
      bacterial genera are contributing to simultaneous polyphosphate accumulation and 
      denitrification processes in the anoxic zones of NDBEPR systems.
FAU - Atkinson, B W
AU  - Atkinson BW
AD  - Centre for Water and Wastewater Research, Technikon Natal, PO Box 953, Durban, 
      4000, South Africa.
FAU - Mudaly, D D
AU  - Mudaly DD
FAU - Bux, F
AU  - Bux F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA, Bacterial)
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
SB  - IM
MH  - DNA, Bacterial/genetics
MH  - Hypoxia
MH  - In Situ Hybridization, Fluorescence
MH  - Phosphorus/*metabolism
MH  - Population Dynamics
MH  - Pseudomonas/*physiology
MH  - Sewage/*microbiology
MH  - Waste Disposal, Fluid
EDAT- 2001/05/31 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/05/31 10:00
PHST- 2001/05/31 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/05/31 10:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2001;43(1):139-46.