PMID- 11379113
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 43
IP  - 1
DP  - 2001
TI  - Population dynamics of anaerobic microbial consortia in thermophilic granular 
      sludge in response to feed composition change.
PG  - 59-66
AB  - A thermophilic UASB reactor was operated at 55 degrees C for greater than 470 
      days in order to investigate the effects of feed composition on the changes in 
      microbial community structure where thermophilic granular sludge was used as the 
      inoculum source. The feed compositions were changed with cultivation days; phase 
      1 (1-70 days), alcohol distillery wastewater; phase 2 (71-281 days), artificial 
      acetate wastewater; phase 3 (282-474 days), artificial sucrose wastewater. During 
      the first one month of each phase, the methanogenic activity and cell density of 
      methanogens quantified by fluorescence in situ hybridization (FISH) drastically 
      changed as a result of shift in feed composition. When artificial acetate 
      wastewater was used as feed, retained granular sludge was partially disintegrated 
      due to a decrease in the number of symbiotic bacterial community members: 
      acetogens (acidogens) and hydrogenotrophic methanogens. In contrast, when the 
      feed was shifted to sucrose (phase 3), granulation of biomass was promoted by a 
      remarkable proliferation of the symbiotic community. The presence of 
      hydrogen-utilizing methanogens and acetogens (acidogens) are shown to be 
      effective for the enhancement of thermophilic granulation. The cell density of 
      methanogens determined by FISH was strongly correlated with the methane-producing 
      potential of the retained thermophilic granular sludge.
FAU - Syutsubo, K
AU  - Syutsubo K
AD  - Kamaishi Laboratories, Marine Biotechnology Institute Co., Ltd., Heita, Kamaishi, 
      Iwate 026-0001, Japan.
FAU - Sinthurat, N
AU  - Sinthurat N
FAU - Ohashi, A
AU  - Ohashi A
FAU - Harada, H
AU  - Harada H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (Acetates)
RN  - 0 (Sewage)
RN  - 3K9958V90M (Ethanol)
RN  - 57-50-1 (Sucrose)
SB  - IM
MH  - Acetates/metabolism
MH  - *Bacteria, Anaerobic
MH  - Biomass
MH  - Ethanol/metabolism
MH  - Euryarchaeota
MH  - In Situ Hybridization, Fluorescence
MH  - Population Dynamics
MH  - *Refuse Disposal
MH  - Sewage/*microbiology
MH  - Sucrose/metabolism
MH  - Temperature
EDAT- 2001/05/31 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/05/31 10:00
PHST- 2001/05/31 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/05/31 10:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2001;43(1):59-66.