PMID- 11379772
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 24
IP  - 5
DP  - 2001 May
TI  - Cytotoxicity of reactive oxygen species and related agents toward 
      undifferentiated and differentiated rat phenochromocytoma PC12 cells.
PG  - 515-9
AB  - The cytotoxicity of reactive oxygen species and related agents toward cultured 
      rat adrenal medullary phenochromocytoma PC12 cells was examined. These species 
      and agents include hydrogen peroxide, linoleic acid hydroperoxide (LOOH), 
      tert-butyl hydroperoxide, paraquat, 2,2'-azobis(2-amidinopropane) dihydrochloride 
      (AAPH), 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN), and a 
      hypoxanthine-xanthine oxidase system. The respective 50% lethal concentrations 
      (LC50) for undifferentiated and differentiated PC12 cells were found to be 275 
      and 165 microM of hydrogen peroxide, 58.3 and 35.3 microM of LOOH, 536 and 212 
      microM of tert-butyl hydroperoxide, 42.5 and 26.5 mM of paraquat, 79.5 and 74.5 
      mM of AAPH, 412 and 300 microM of AMVN, and 37.2 and 16.6mU x ml(-1) xanthine 
      oxidase activity of the hypoxanthine-xanthine oxidase system. These results show 
      that the differentiated cells were more susceptible to these oxidative agents 
      than the undifferentiated cells. The glutathione peroxidase activity level of the 
      undifferentiated cells was 2-3 times higher than the differentiated cells, the 
      catalase activity level also tended to be higher, the superoxide dismutase 
      activity level was higher on a per-protein-quantity basis but lower on a 
      per-cell-number basis, and the total and reduced glutathione concentration levels 
      were considerably higher. The enhanced susceptibility of the differentiated cells 
      may result from decreases in the activity of glutathione peroxidase and the 
      concentration of its substrate, reduced glutathione (GSH). Further, the 
      preincubation of PC12 cells with alpha-tocopherol or 
      L-buthionine-(R,S)-sulfoximine (BSO) lowered or enhanced their cytotoxicities, 
      respectively.
FAU - Sasaki, N
AU  - Sasaki N
AD  - Department of Biology, Faculty of Science and High Technology Research Center, 
      Konan University, Kobe, Japan.
FAU - Baba, N
AU  - Baba N
FAU - Matsuo, M
AU  - Matsuo M
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Linoleic Acids)
RN  - 0 (Lipid Peroxides)
RN  - 0 (Reactive Oxygen Species)
RN  - 11062-77-4 (Superoxides)
RN  - 1406-18-4 (Vitamin E)
RN  - 25657-09-4 (linoleic acid hydroperoxide)
RN  - 5072-26-4 (Buthionine Sulfoximine)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Buthionine Sulfoximine/pharmacology
MH  - Cell Differentiation
MH  - Cell Survival/*drug effects
MH  - Hydrogen Peroxide/toxicity
MH  - Linoleic Acids/toxicity
MH  - Lipid Peroxides/toxicity
MH  - PC12 Cells
MH  - Rats
MH  - *Reactive Oxygen Species
MH  - Superoxides/metabolism
MH  - Vitamin E/pharmacology
EDAT- 2001/05/31 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/05/31 10:00
PHST- 2001/05/31 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/05/31 10:00 [entrez]
AID - 10.1248/bpb.24.515 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2001 May;24(5):515-9. doi: 10.1248/bpb.24.515.