PMID- 11379889
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20170214
IS  - 0022-0345 (Print)
IS  - 0022-0345 (Linking)
VI  - 80
IP  - 3
DP  - 2001 Mar
TI  - Mitogenic effects of neutrophins on a periodontal ligament cell line.
PG  - 881-6
AB  - Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and 
      neurotrophin-3 (NT-3) are three representative neurotrophins responsible for the 
      differentiation and survival of neurons, and their high-affinity receptors are 
      tropomyosin-receptor-kinase (TRK)A, TRKB, and TRKC, respectively. In this study, 
      we investigated the expression of neurotrophins in a mouse periodontal ligament 
      cell line (MPL), by reverse transcription-polymerase chain-reaction (RT-PCR) and 
      enzyme-linked immunoabsorbent assay (ELISA). We also studied the expression of 
      TRK receptors on MPL by immunostaining and the effects of neurotrophins on the 
      proliferation of MPL, with a hypothesis of autocrine mechanism of neurotrophins. 
      Each neurotrophin and TRK receptor was expressed, and neurotrophins enhanced the 
      proliferation of MPL. These findings suggest that the MPL has functional 
      neurotrophin receptors involved in an autocrine function of neurotrophins. The 
      expression level of neurotrophins and TRKs showed the reverse pattern, and we 
      propose an auto-regulatory mechanism of ligands and receptors in accordance with 
      the level of synthesized neurotrophins.
FAU - Tsuboi, Y
AU  - Tsuboi Y
AD  - Department of Orthodontics, Okayama University Dental School, Japan.
FAU - Nakanishi, T
AU  - Nakanishi T
FAU - Takano-Yamamoto, T
AU  - Takano-Yamamoto T
FAU - Miyamoto, M
AU  - Miyamoto M
FAU - Yamashiro, T
AU  - Yamashiro T
FAU - Takigawa, M
AU  - Takigawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Coloring Agents)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Mitogens)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/analysis/genetics
MH  - Analysis of Variance
MH  - Animals
MH  - Autocrine Communication
MH  - Brain-Derived Neurotrophic Factor/analysis/genetics
MH  - Cell Differentiation/physiology
MH  - Cell Line
MH  - Cell Survival/physiology
MH  - Coloring Agents
MH  - Culture Media, Conditioned
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Regulation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mitogens/*analysis/genetics
MH  - Nerve Growth Factor/analysis/genetics
MH  - Nerve Growth Factors/*analysis/genetics
MH  - Neurons/metabolism
MH  - Neurotrophin 3/analysis/genetics
MH  - Periodontal Ligament/cytology/*metabolism
MH  - RNA, Messenger/analysis/genetics
MH  - Receptor, trkA/analysis/genetics
MH  - Receptor, trkB/analysis/genetics
MH  - Receptor, trkC/analysis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Statistics as Topic
EDAT- 2001/05/31 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/05/31 10:00
PHST- 2001/05/31 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/05/31 10:00 [entrez]
AID - 10.1177/00220345010800030701 [doi]
PST - ppublish
SO  - J Dent Res. 2001 Mar;80(3):881-6. doi: 10.1177/00220345010800030701.