PMID- 11380546
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20190721
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 44
IP  - 6
DP  - 2001 Jun
TI  - The suitability of hexyl cinnamic aldehyde as a calibrant for the murine local 
      lymph node assay.
PG  - 357-61
AB  - The murine local lymph node assay (LLNA) for the prospective identification of 
      contact allergens assesses skin sensitization potential as a function of 
      proliferative activity induced in lymph nodes draining the site of topical 
      exposure to test chemical. This method has been endorsed recently as a stand 
      alone test for the identification of contact allergens. We have now examined the 
      suitability of hexyl cinnamic aldehyde (HCA), a recommended positive control for 
      skin sensitization testing, as a calibrant for comparing the consistency of LLNA 
      responses with time, and between laboratories, and thus for the routine 
      assessment of assay reliability. Standard LLNAs were performed with CBA strain 
      mice in 3 independent laboratories over a period of 8 years. Dose-response curves 
      were used to derive mathematically the EC3 value (the estimated concentration of 
      chemical necessary to cause a stimulation index (SI) of 3 compared with 
      proliferation induced by concurrent vehicle controls). In each laboratory, 6 
      separate experiments were conducted using a single concentration of HCA (25%). 
      Very similar stimulation indices were achieved, with mean values of 9.0, 6.5 and 
      6.6 recorded. A total of 10 dose-response experiments were performed 
      independently in the 3 laboratories and these revealed that there was very little 
      inter-laboratory, or temporal, variation in EC3 values. These data confirm that 
      HCA responses in the LLNA are very stable and demonstrate that HCA provides a 
      suitable calibrant for determining assay sensitivity and performance.
FAU - Dearman, R J
AU  - Dearman RJ
AD  - Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, 
      SK10 4TJ, UK.
FAU - Wright, Z M
AU  - Wright ZM
FAU - Basketter, D A
AU  - Basketter DA
FAU - Ryan, C A
AU  - Ryan CA
FAU - Gerberick, G F
AU  - Gerberick GF
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Allergens)
RN  - 101-86-0 (hexyl cinnamic aldehyde)
RN  - 7864XYD3JJ (Acrolein)
RN  - 958-74-7 (methylthymidine)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Acrolein/*adverse effects/*analogs & derivatives
MH  - Allergens/*adverse effects
MH  - Animals
MH  - Dermatitis, Allergic Contact/diagnosis/etiology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Lymph Nodes/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Reproducibility of Results
MH  - Thymidine/*analogs & derivatives/metabolism
EDAT- 2001/05/31 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/05/31 10:00
PHST- 2001/05/31 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/05/31 10:00 [entrez]
AID - cod440607 [pii]
AID - 10.1034/j.1600-0536.2001.044006357.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 2001 Jun;44(6):357-61. doi: 
      10.1034/j.1600-0536.2001.044006357.x.