PMID- 11382013
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20131121
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 41
IP  - 3
DP  - 2000
TI  - Microbial ecology of the leach bed anaerobic digestion of unsorted municipal 
      solid waste.
PG  - 9-16
AB  - The microbial ecology of the sequential, leach-bed, mesophilic anaerobic 
      digestion of unsorted, coarse municipal solid waste (MSW) was examined over 80 
      days. The methane yield was approximately 75% of the ultimate biochemical methane 
      potential (BMP) of the waste loaded into the digesters. The operational strategy 
      involved a sequence of two digesters containing fresh and anaerobically 
      stabilised MSW respectively. Cell wall phospholipid fatty acid (PLFA) and ether 
      lipid (PLEL) analysis was used to monitor changes in microbial biomass. Both 
      Bacterial and Archaeal biomass were heavily influenced by pH during the two-week 
      start up period. Archaeal biomass peaked just before the methane production rate 
      reached a maximum whereas Bacterial biomass peaked at a later stage. Changes in 
      the phylogenetic diversity of the population were monitored by denaturing 
      gradient gel electrophoresis (DGGE). An analysis of the changes in DGGE banding 
      patterns suggested that rapid start-up of a new reactor was effected by 
      inoculation as well as the provision of buffering capacity from the mature 
      reactor leachate.
FAU - Silvey, P
AU  - Silvey P
AD  - Department of Chemical Engineering, University of Queensland, Brisbane QLD 4072, 
      Australia.
FAU - Pullammanappallil, P C
AU  - Pullammanappallil PC
FAU - Blackall, L
AU  - Blackall L
FAU - Nichols, P
AU  - Nichols P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Ethers)
RN  - 0 (Fatty Acids)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Archaea/metabolism
MH  - Bacteria, Anaerobic/*metabolism
MH  - Biodegradation, Environmental
MH  - Biomass
MH  - Bioreactors/*microbiology
MH  - DNA, Archaeal/chemistry
MH  - DNA, Bacterial/chemistry
MH  - Ethers/analysis
MH  - Fatty Acids/analysis
MH  - Hydrogen-Ion Concentration
MH  - Methane/analysis
MH  - Polymerase Chain Reaction
MH  - Refuse Disposal/*methods
EDAT- 2001/05/31 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/05/31 10:00
PHST- 2001/05/31 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/05/31 10:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2000;41(3):9-16.