PMID- 11382196
OWN - NLM
STAT- MEDLINE
DCOM- 20011011
LR  - 20191104
IS  - 1590-1874 (Print)
IS  - 1590-1874 (Linking)
VI  - 21
IP  - 5 Suppl
DP  - 2000
TI  - Cytogenetic damage in peripheral lymphocytes of mitochondrial disease patients.
PG  - S963-5
AB  - Recent studies indicate an important role of endogenous oxidative stress in the 
      onset and/or in the progression of mitochondrial encephalomyopathies. In 
      particular, the increased production of radical species caused by altered 
      mitochondrial functionality could affect both mitochondrial and nuclear DNA 
      integrity. We performed the micronucleus assay coupled with fluorescence in situ 
      hybridization (FISH) to detect chromosome damage in peripheral blood lymphocytes 
      in a group of patients affected by different forms of mitochondrial 
      encephalomyopathies. Moreover the comet assay has been carried out to detect 
      primary and oxidative damage in the nuclear DNA. Our results show a significant 
      presence of both DNA and chromosome damage in patients compared to a matched 
      group of controls. A reduction in DNA alterations is also observed in patients 
      after treatment with coenzyme-Q10.
FAU - Naccarati, A
AU  - Naccarati A
AD  - Department of Human and Environmental Sciences, University of Pisa, Via S. 
      Giuseppe 22, Pisa, Italy.
FAU - Molinu, S
AU  - Molinu S
FAU - Mancuso, M
AU  - Mancuso M
FAU - Siciliano, G
AU  - Siciliano G
FAU - Migliore, L
AU  - Migliore L
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Neurol Sci
JT  - Neurological sciences : official journal of the Italian Neurological Society and 
      of the Italian Society of Clinical Neurophysiology
JID - 100959175
RN  - 0 (Coenzymes)
RN  - 1339-63-5 (Ubiquinone)
RN  - 9007-49-2 (DNA)
RN  - EJ27X76M46 (coenzyme Q10)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations/*blood/genetics
MH  - Chromosome Disorders
MH  - Coenzymes
MH  - Comet Assay/methods
MH  - DNA/drug effects/metabolism
MH  - DNA Damage/drug effects/*physiology
MH  - DNA Mutational Analysis
MH  - Female
MH  - Humans
MH  - Lymphocytes/drug effects/metabolism/*pathology
MH  - Male
MH  - Micronucleus Tests
MH  - Middle Aged
MH  - Mitochondrial Encephalomyopathies/drug therapy/*genetics/metabolism
MH  - Oxidative Stress/drug effects/*genetics
MH  - Treatment Outcome
MH  - Ubiquinone/analogs & derivatives/therapeutic use
EDAT- 2001/05/31 10:00
MHDA- 2001/10/12 10:01
CRDT- 2001/05/31 10:00
PHST- 2001/05/31 10:00 [pubmed]
PHST- 2001/10/12 10:01 [medline]
PHST- 2001/05/31 10:00 [entrez]
AID - 10.1007/s100720070010 [doi]
PST - ppublish
SO  - Neurol Sci. 2000;21(5 Suppl):S963-5. doi: 10.1007/s100720070010.