PMID- 11386836
OWN - NLM
STAT- MEDLINE
DCOM- 20011025
LR  - 20061115
IS  - 0967-5868 (Print)
IS  - 0967-5868 (Linking)
VI  - 8 Suppl 1
DP  - 2001 May
TI  - Microcirculatory alterations in a Mongolian gerbil sinus-vein thrombosis model.
PG  - 97-105
AB  - BACKGROUND AND PURPOSE: The pathophysiology of sinus vein thrombosis (SVT) is 
      still controversial in patients and experimental animals, the microcirculatory 
      alterations in particular. This study was designed to develop a new sinus vein 
      thrombosis model and to further elucidate pathophysiological events such as the 
      relationship between local and regional cerebral blood flow and haemoglobin 
      oxygen saturation (HbSO2), changes of the microvasculature, leukocyte behaviour 
      and brain tissue damage. METHODS: In a first experimental series, animals were 
      divided into two groups which resulted from different procedures of inducing SVT. 
      In the SSS middle occlusion group (SMO group), SVT was induced by the ligation of 
      the superior sagittal sinus right in the middle between the bregma and the 
      confluence sinum. In the SSS posterior occlusion group (SPO group) the ligation 
      was performed close to the confluence sinum. Regional cerebral blood flow (rCBF) 
      was assessed at 36 identical locations by laser Doppler flowmetry together with 
      regional haemoglobin oxygen saturation (HbSO2). In a second series of experiments 
      SVT was induced by ligation of the SSS close to the confluence sinum (SVT group) 
      to study effects on the cortical microcirculation. A sham operation was performed 
      in six animals (sham group). In both groups, an intravital microscopic double 
      tracing technique was utilised for evaluating microvessel structures and 
      leukocyte behaviour. The images were recorded on videotape for evaluating 
      alterations of microvessel (venules, arterioles and capillaries) diameters and 
      numbers of leukocyte rollers and stickers by a digital video analyser. Animals 
      were sacrificed for histological evaluation after 5 days. RESULTS: The posterior 
      sinus ligation caused a significant decrease of rCBF and HbSO2 and brain tissue 
      damage which was not seen in the SMO group. Alteration of rCBF and HbSO2 were 
      positively correlated with infarct size in the SPO group only, where venous 
      infarction was easily reproduced. Therefore, it is suggested that this model is 
      suitable for studying SVT in Mongolian gerbils. Intravital microscopy of the 
      cortical microcirculation revealed no significant changes of vessels diameter in 
      the sham group, whereas a significant dilation of veins and capillaries was seen 
      in the SVT group. Numbers of leukocyte rollers and stickers were positively 
      correlated with infarct size. CONCLUSION: Microcirculatory alterations and brain 
      tissue damage from SVT in the Mongolian gerbil depend on the SSS occlusion site. 
      The newly established mongolian gerbil sinus-vein thrombosis model has advantages 
      compared to previously reported sinus-vein thrombosis models such as easy 
      handling, easy technique, highly reproducibility, and good observation of 
      microcirculatory event. The model allows for studies of cerebral low-flow 
      conditions such as expected to occur in an ischaemic penumbra zone.
CI  - Copyright 2001 Harcourt Publishers Ltd.
FAU - Miyamoto, K
AU  - Miyamoto K
AD  - Department of Neurosurgery, Nara Medical University, Japan.
FAU - Heimann, A
AU  - Heimann A
FAU - Kempski, O
AU  - Kempski O
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Scotland
TA  - J Clin Neurosci
JT  - Journal of clinical neuroscience : official journal of the Neurosurgical Society 
      of Australasia
JID - 9433352
SB  - IM
MH  - Animals
MH  - Cerebral Infarction/etiology/*physiopathology
MH  - Cerebral Veins/*pathology
MH  - *Cerebrovascular Circulation
MH  - Gerbillinae
MH  - Laser-Doppler Flowmetry
MH  - Ligation
MH  - Male
MH  - Microcirculation
MH  - *Models, Animal
MH  - Reproducibility of Results
MH  - Sinus Thrombosis, Intracranial/complications/*physiopathology
MH  - Species Specificity
MH  - Venous Thrombosis/complications/*physiopathology
EDAT- 2001/06/02 10:00
MHDA- 2001/10/26 10:01
CRDT- 2001/06/02 10:00
PHST- 2001/06/02 10:00 [pubmed]
PHST- 2001/10/26 10:01 [medline]
PHST- 2001/06/02 10:00 [entrez]
AID - S0967586801908872 [pii]
AID - 10.1054/jocn.2001.0887 [doi]
PST - ppublish
SO  - J Clin Neurosci. 2001 May;8 Suppl 1:97-105. doi: 10.1054/jocn.2001.0887.