PMID- 11387026
OWN - NLM
STAT- MEDLINE
DCOM- 20010726
LR  - 20190518
IS  - 0261-1929 (Print)
IS  - 0261-1929 (Linking)
VI  - 29
IP  - 3
DP  - 2001 May-Jun
TI  - Protein precipitation In Vitro as a measure of chemical-induced cytotoxicity: an 
      EDIT sub-programme.
PG  - 309-24
AB  - As a priority area of the Evaluation-Guided Development of In Vitro Toxicity and 
      Toxicokinetic Tests (EDIT) programme, an in vitro protein precipitation (PP) 
      assay was used on the 50 reference chemicals of the Multicentre Evaluation of In 
      Vitro Cytotoxicity (MEIC) project, to confirm and extend the MEIC results. 
      Dose-response curves were generated for only 30 of the chemicals, and the 
      concentrations causing 10% (EC10) and 50% (EC50) protein precipitation versus the 
      positive control were chosen as endpoints. The number of chemicals with a 
      positive response increased to 46 when a new endpoint, the minimum effect 
      concentration (MEC) that induces protein precipitation with respect to the 
      negative control, was used. When the results were correlated with in vitro 
      cytotoxicity in human cell lines, a similarly good correlation was found between 
      the various endpoints of the PP assay at 5 hours and the 24-hour IC50 average 
      cytotoxicity in human cell lines, even though the number of chemicals included in 
      the correlation was larger for the MEC. Using the prediction error, the endpoint 
      that gave the best correlation between the PP assay and human cell cytotoxicity 
      was once more found to be the 5-hour MEC, and this was chosen for the PP assay. 
      The sensitivity of the PP assay is lower than that of the in vitro cell-line 
      cytotoxicity assay, possibly due to its shorter exposure period and because 
      precipitation is the ultimate event in the sequence of a protein disturbance. It 
      is expected that earlier denaturation steps would give better sensitivity. 
      However, this simple, inexpensive and rapid assay could be useful in the early 
      stages of testing chemicals.
FAU - Novillo, A
AU  - Novillo A
AD  - Environmental Toxicology, CISA-INIA, Valdeolmos 28130, Madrid, Spain.
FAU - Ekwall, B
AU  - Ekwall B
FAU - Castano, A
AU  - Castano A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Altern Lab Anim
JT  - Alternatives to laboratory animals : ATLA
JID - 8110074
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Cell Line
MH  - Chemical Precipitation
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - In Vitro Techniques
MH  - Inhibitory Concentration 50
MH  - Ovalbumin/*chemistry
MH  - Toxicity Tests/*methods
EDAT- 2001/06/02 10:00
MHDA- 2001/07/28 10:01
CRDT- 2001/06/02 10:00
PHST- 2001/06/02 10:00 [pubmed]
PHST- 2001/07/28 10:01 [medline]
PHST- 2001/06/02 10:00 [entrez]
AID - 10.1177/026119290102900315 [doi]
PST - ppublish
SO  - Altern Lab Anim. 2001 May-Jun;29(3):309-24. doi: 10.1177/026119290102900315.