PMID- 11387292
OWN - NLM
STAT- MEDLINE
DCOM- 20010823
LR  - 20190513
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 16
IP  - 6
DP  - 2001 Jun
TI  - Evidence of reduced single-stranded testicular sperm DNA from obstructive 
      azoospermic men after 3 days of in-vitro culture.
PG  - 1200-3
AB  - The aim of the present study was to verify whether culturing testicular tissue, 
      to obtain a higher percentage of motile spermatozoa and a better post-thaw 
      recovery rate, affected the ratio between single/double-stranded sperm DNA and, 
      consequently, DNA sensitivity to damage. Testicular biopsy samples from men with 
      obstructive and secretory azoospermia, candidates for assisted reproductive 
      treatment, were cultured for 72 h. The percentage of motile spermatozoa and the 
      single/double stranded DNA ratio were assessed on the day of retrieval (day 0) 
      and again on day 3. The single/double stranded DNA ratio was measured by the 
      acridine orange (AO) staining method. Spermatozoa were classified as green 
      (double-stranded chromatin) or red fluorescing (single-stranded chromatin). In 
      obstructive azoospermia, median motility was 22% (range 10-44%) on day 0 and 50% 
      (range 38-63%) on day 3 (P < 0.01). The median percentage of red stained 
      spermatozoa was 53.5% (range 0.1-88%) on day 0 and 20% (range 2.7-99.9%) on day 3 
      (P < 0.05). No changes were observed in secretory azoospermia. The culture 
      procedure from obstructive azoospermia not only increased the post-thaw recovery 
      rate, as previously observed, but also reduced the portion of spermatozoa 
      containing single-stranded DNA, thereby increasing the availability of 
      double-stranded DNA spermatozoa for ICSI use.
FAU - Emiliani, S
AU  - Emiliani S
AD  - Fertility Clinic and Laboratory of Biology and Psychology of Human Fertility, 
      Erasmus Hospital French Speaking Free University of Brussels, 808, Route de 
      Lennik, B1070 Brussels, Belgium. semilian@ulb.ac.be
FAU - Van Den Bergh, M
AU  - Van Den Bergh M
FAU - Vannin, A S
AU  - Vannin AS
FAU - Biramane, J
AU  - Biramane J
FAU - Verdoodt, M
AU  - Verdoodt M
FAU - Englert, Y
AU  - Englert Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (DNA, Single-Stranded)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Biopsy
MH  - Cells, Cultured
MH  - DNA Damage
MH  - DNA, Single-Stranded/*analysis
MH  - Humans
MH  - Male
MH  - Oligospermia/pathology/*physiopathology
MH  - Sperm Count
MH  - Sperm Motility
MH  - Spermatozoa/*chemistry
MH  - Staining and Labeling
MH  - Testis/*pathology
MH  - Time Factors
EDAT- 2001/06/02 10:00
MHDA- 2001/08/24 10:01
CRDT- 2001/06/02 10:00
PHST- 2001/06/02 10:00 [pubmed]
PHST- 2001/08/24 10:01 [medline]
PHST- 2001/06/02 10:00 [entrez]
AID - 10.1093/humrep/16.6.1200 [doi]
PST - ppublish
SO  - Hum Reprod. 2001 Jun;16(6):1200-3. doi: 10.1093/humrep/16.6.1200.