PMID- 11389757
OWN - NLM
STAT- MEDLINE
DCOM- 20010913
LR  - 20231213
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 26
IP  - 2
DP  - 2001 Apr
TI  - The 5' untranslated region of the At-P5R gene is involved in both transcriptional 
      and post-transcriptional regulation.
PG  - 157-69
AB  - The steady-state level of transcripts coding for the pyrroline-5-carboxylate 
      reductase of Arabidopsis (At-P5R) increased under salt and heat stress, mainly 
      because of an enhanced mRNA stability. However, the At-P5R protein level was not 
      induced, and its translation was inhibited at initiation stage and probably also 
      at later stages. Replacement of the 5' untranslated region (5'UTR) and 
      beta-glucuronidase (gus) fusion analysis revealed that the first 92 bp region of 
      the At-P5R 5'UTR was sufficient to mediate transcript stabilization and 
      translation inhibition during salt and heat stresses. Furthermore, the first 92 
      bp region of the At-P5R 5'UTR was also involved in transcription efficiency in a 
      promoter-dependent manner. The results demonstrated that the stress regulation of 
      At-P5R is complex and involves the 5'UTR which acts at three levels, partly in 
      opposing directions.
FAU - Hua, X J
AU  - Hua XJ
AD  - Vakgroep Moleculaire Genetica & Departement Plantengenetica, Vlaams 
      Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. 
      Ledeganckstraat 35, B-9000 Gent, Belgium.
FAU - Van de Cotte, B
AU  - Van de Cotte B
FAU - Van Montagu, M
AU  - Van Montagu M
FAU - Verbruggen, N
AU  - Verbruggen N
LA  - eng
SI  - GENBANK/Y08951
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA primers)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Plant)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 63231-63-0 (RNA)
RN  - EC 1.5.1.- (Pyrroline Carboxylate Reductases)
RN  - EC 3.2.1.31 (Glucuronidase)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Arabidopsis/*genetics
MH  - Base Sequence
MH  - Blotting, Northern
MH  - *Gene Expression Regulation, Enzymologic
MH  - *Gene Expression Regulation, Plant
MH  - Glucuronidase/genetics
MH  - Hot Temperature
MH  - Molecular Sequence Data
MH  - Plant Proteins/*genetics
MH  - Polyribosomes/genetics
MH  - Promoter Regions, Genetic
MH  - Protein Biosynthesis
MH  - Pyrroline Carboxylate Reductases/*genetics
MH  - RNA
MH  - RNA Processing, Post-Transcriptional
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Plant
MH  - Recombinant Fusion Proteins
MH  - Sodium Chloride
MH  - Transcription Factors/metabolism
MH  - Transcription, Genetic
MH  - delta-1-Pyrroline-5-Carboxylate Reductase
EDAT- 2001/06/08 10:00
MHDA- 2001/09/14 10:01
CRDT- 2001/06/08 10:00
PHST- 2001/06/08 10:00 [pubmed]
PHST- 2001/09/14 10:01 [medline]
PHST- 2001/06/08 10:00 [entrez]
AID - tpj1020 [pii]
AID - 10.1046/j.1365-313x.2001.01020.x [doi]
PST - ppublish
SO  - Plant J. 2001 Apr;26(2):157-69. doi: 10.1046/j.1365-313x.2001.01020.x.