PMID- 11390355
OWN - NLM
STAT- MEDLINE
DCOM- 20010705
LR  - 20220227
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 15
IP  - 11
DP  - 2001 Jun 1
TI  - A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome 
      pairing and disjunction.
PG  - 1349-60
AB  - We have studied four Caenorhabditis elegans homologs of the Rad21/Scc1/Rec8 
      sister-chromatid cohesion protein family. Based on the RNAi phenotype and protein 
      localization, it is concluded that one of them, W02A2.6p, is the likely worm 
      ortholog of yeast Rec8p. The depletion of C. elegans W02A2.6p (called REC-8) by 
      RNAi, induced univalent formation and splitting of chromosomes into sister 
      chromatids at diakinesis. Chromosome synapsis at pachytene was defective, but 
      primary homology recognition seemed unaffected, as a closer-than-random 
      association of homologous fluorescence in situ hybridization (FISH) signals at 
      leptotene/zygotene was observed. Depletion of REC-8 also induced chromosome 
      fragmentation at diakinesis. We interpret these fragments as products of 
      unrepaired meiotic double-stranded DNA breaks (DSBs), because fragmentation was 
      suppressed in a spo-11 background. Thus, REC-8 seems to be required for 
      successful repair of DSBs. The occurrence of DSBs in REC-8-depleted meiocytes 
      suggests that DSB formation does not depend on homologous synapsis. Anti-REC-8 
      immunostaining decorated synaptonemal complexes (SCs) at pachytene and 
      chromosomal axes in bivalents and univalents at diakinesis. Between metaphase I 
      and metaphase II, REC-8 is partially lost from the chromosomes. The partial loss 
      of REC-8 from chromosomes between metaphase I and metaphase II suggests that worm 
      REC-8 might function similarly to yeast Rec8p. The loss of yeast Rec8p from 
      chromosome arms at meiosis I and centromeres at meiosis II coordinates the 
      disjunction of homologs and sister chromatids at the two meiotic divisions.
FAU - Pasierbek, P
AU  - Pasierbek P
AD  - Department of Cytology and Genetics, Institute of Botany, University of Vienna, 
      A-1030 Vienna, Austria.
FAU - Jantsch, M
AU  - Jantsch M
FAU - Melcher, M
AU  - Melcher M
FAU - Schleiffer, A
AU  - Schleiffer A
FAU - Schweizer, D
AU  - Schweizer D
FAU - Loidl, J
AU  - Loidl J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (Fungal Proteins)
RN  - 0 (Helminth Proteins)
RN  - 0 (MCD1 protein, S cerevisiae)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Schizosaccharomyces pombe Proteins)
RN  - 0 (scc-1 protein, C elegans)
RN  - 148813-46-1 (REC8 protein, S pombe)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/*cytology/genetics
MH  - *Caenorhabditis elegans Proteins
MH  - Cell Cycle Proteins/analysis/*genetics/metabolism
MH  - Chromatids/genetics
MH  - Chromosomal Proteins, Non-Histone
MH  - Chromosome Segregation/*genetics
MH  - Conserved Sequence
MH  - Fluorescent Antibody Technique
MH  - Fungal Proteins/*genetics
MH  - Helminth Proteins/analysis/*genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Meiosis/*genetics
MH  - Nuclear Proteins/analysis/*genetics/metabolism
MH  - Phosphoproteins/analysis/*genetics/metabolism
MH  - Phylogeny
MH  - Saccharomyces cerevisiae Proteins
MH  - *Schizosaccharomyces pombe Proteins
MH  - Synaptonemal Complex/genetics/metabolism
PMC - PMC312707
EDAT- 2001/06/08 10:00
MHDA- 2001/07/06 10:01
PMCR- 2001/12/01
CRDT- 2001/06/08 10:00
PHST- 2001/06/08 10:00 [pubmed]
PHST- 2001/07/06 10:01 [medline]
PHST- 2001/06/08 10:00 [entrez]
PHST- 2001/12/01 00:00 [pmc-release]
AID - 10.1101/gad.192701 [doi]
PST - ppublish
SO  - Genes Dev. 2001 Jun 1;15(11):1349-60. doi: 10.1101/gad.192701.