PMID- 11390668
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20181113
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 21
IP  - 13
DP  - 2001 Jul
TI  - Ligand-mediated assembly and real-time cellular dynamics of estrogen receptor 
      alpha-coactivator complexes in living cells.
PG  - 4404-12
AB  - Studies with live cells demonstrate that agonist and antagonist rapidly (within 
      minutes) modulate the subnuclear dynamics of estrogen receptor alpha (ER) and 
      steroid receptor coactivator 1 (SRC-1). A functional cyan fluorescent protein 
      (CFP)-tagged lac repressor-ER chimera (CFP-LacER) was used in live cells to 
      discretely immobilize ER on stably integrated lac operator arrays to study 
      recruitment of yellow fluorescent protein (YFP)-steroid receptor coactivators 
      (YFP-SRC-1 and YFP-CREB binding protein [CBP]). In the absence of ligand, 
      YFP-SRC-1 is found dispersed throughout the nucleoplasm, with a surprisingly high 
      accumulation on the CFP-LacER arrays. Agonist addition results in the rapid 
      (within minutes) recruitment of nucleoplasmic YFP-SRC-1, while antagonist 
      additions diminish YFP-SRC-1-CFP-LacER associations. Less ligand-independent 
      colocalization is observed with CFP-LacER and YFP-CBP, but agonist-induced 
      recruitment occurs within minutes. The agonist-induced recruitment of 
      coactivators requires helix 12 and critical residues in the ER-SRC-1 interaction 
      surface, but not the F, AF-1, or DNA binding domains. Fluorescence recovery after 
      photobleaching indicates that YFP-SRC-1, YFP-CBP, and CFP-LacER complexes undergo 
      rapid (within seconds) molecular exchange even in the presence of an agonist. 
      Taken together, these data suggest a dynamic view of receptor-coregulator 
      interactions that is now amenable to real-time study in living cells.
FAU - Stenoien, D L
AU  - Stenoien DL
AD  - Department of Molecular and Cellular Biology, Baylor College of Medicine, 
      Houston, Texas 77030, USA.
FAU - Nye, A C
AU  - Nye AC
FAU - Mancini, M G
AU  - Mancini MG
FAU - Patel, K
AU  - Patel K
FAU - Dutertre, M
AU  - Dutertre M
FAU - O'Malley, B W
AU  - O'Malley BW
FAU - Smith, C L
AU  - Smith CL
FAU - Belmont, A S
AU  - Belmont AS
FAU - Mancini, M A
AU  - Mancini MA
LA  - eng
GR  - T32-GM07283/GM/NIGMS NIH HHS/United States
GR  - R01-DK55622/DK/NIDDK NIH HHS/United States
GR  - R01 DK53002/DK/NIDDK NIH HHS/United States
GR  - R01 DK055622/DK/NIDDK NIH HHS/United States
GR  - R01 GM042516/GM/NIGMS NIH HHS/United States
GR  - R01 DK053002/DK/NIDDK NIH HHS/United States
GR  - R01-GM58460/GM/NIGMS NIH HHS/United States
GR  - F32 DK009787/DK/NIDDK NIH HHS/United States
GR  - T32 GM007283/GM/NIGMS NIH HHS/United States
GR  - R01 GM058460/GM/NIGMS NIH HHS/United States
GR  - 1F32DK09787/DK/NIDDK NIH HHS/United States
GR  - R01-GM42516/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogens)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Amino Acid Motifs
MH  - Animals
MH  - Binding Sites
MH  - Cell Line
MH  - Estrogen Receptor alpha
MH  - Estrogens/pharmacology
MH  - Genes, Reporter
MH  - Histone Acetyltransferases
MH  - Lac Operon/*genetics
MH  - Ligands
MH  - Microscopy, Fluorescence
MH  - Nuclear Receptor Coactivator 1
MH  - Protein Binding/drug effects
MH  - Receptors, Estrogen/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Regulatory Sequences, Nucleic Acid/genetics
MH  - Trans-Activators/genetics/metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Transfection
PMC - PMC87100
EDAT- 2001/06/08 10:00
MHDA- 2001/07/20 10:01
PMCR- 2001/07/01
CRDT- 2001/06/08 10:00
PHST- 2001/06/08 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/06/08 10:00 [entrez]
PHST- 2001/07/01 00:00 [pmc-release]
AID - 0162 [pii]
AID - 10.1128/MCB.21.13.4404-4412.2001 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2001 Jul;21(13):4404-12. doi: 10.1128/MCB.21.13.4404-4412.2001.