PMID- 11391201
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20190713
IS  - 0032-1052 (Print)
IS  - 0032-1052 (Linking)
VI  - 107
IP  - 7
DP  - 2001 Jun
TI  - Ontogeny of expression of transforming growth factor-beta 1 (TGF-beta 1), 
      TGF-beta 3, and TGF-beta receptors I and II in fetal rat fibroblasts and skin.
PG  - 1787-94; discussion 1795-6
AB  - Fetal cutaneous wounds that occur in early gestation heal without scar formation. 
      Although much work has been done to characterize the role of transforming growth 
      factor-beta (TGF-beta) isoforms in the adult wound repair process, their function 
      in fetal scarless wound repair is not well understood. The authors hypothesized 
      that the pattern of expression for TGF-beta isoforms and their receptors may 
      influence the phenotypic transition from scarless to scar-forming repair observed 
      during fetal gestation. Using time-dated fetal Sprague-Dawley rat fibroblasts and 
      unwounded skin at gestational ages 14, 16, 18, and 21 days postcoitum of the 
      scarless (< or =16 days) and scar-forming (>16 days) periods of gestation (term = 
      21.5 days), the authors analyzed the endogenous messenger RNA (mRNA) levels of 
      TGF-beta 1 and TGF-beta 3 and their signaling receptors TGF-beta-RI and 
      TGF-beta-RII. Northern blot analyses in both fibroblasts and unwounded skin 
      revealed that levels of TGF-beta 1 were not differentially expressed, whereas 
      more TGF-beta 3 mRNA transcript was found in early than in late gestation. 
      Fibroblast expression of TGF-beta-RI showed no substantial differences, whereas 
      expression of TGF-beta-RII increased during gestation. In contrast, expression of 
      both TGF-beta-RI and TGF-beta-RII in unwounded skin showed decreasing levels as a 
      function of gestational age. The differential levels of TGF-beta 1 and TGF-beta 3 
      suggest that the ratio of these cytokines may provide a predominantly 
      antiscarring or profibrotic signal upon wounding during the scar-free or 
      scar-forming periods of gestation, respectively. Furthermore, lower amounts of 
      the ligand-binding TGF-beta-RII seen in early gestation fibroblasts suggest a 
      decreased ability to perceive ligand during the period of scarless repair.
FAU - Hsu, M
AU  - Hsu M
AD  - Department of Surgery, Stanford University School of Medicine, Stanford, CA 
      94305, USA.
FAU - Peled, Z M
AU  - Peled ZM
FAU - Chin, G S
AU  - Chin GS
FAU - Liu, W
AU  - Liu W
FAU - Longaker, M T
AU  - Longaker MT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Plast Reconstr Surg
JT  - Plastic and reconstructive surgery
JID - 1306050
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Tgfb3 protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Transforming Growth Factor beta3)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Fibroblasts/*metabolism
MH  - Gene Expression
MH  - Gestational Age
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Receptors, Transforming Growth Factor beta
MH  - Skin/*embryology/metabolism
MH  - Transforming Growth Factor beta/*metabolism
MH  - Transforming Growth Factor beta1
MH  - Transforming Growth Factor beta3
EDAT- 2001/06/08 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/06/08 10:00
PHST- 2001/06/08 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/06/08 10:00 [entrez]
AID - 10.1097/00006534-200106000-00023 [doi]
PST - ppublish
SO  - Plast Reconstr Surg. 2001 Jun;107(7):1787-94; discussion 1795-6. doi: 
      10.1097/00006534-200106000-00023.