PMID- 11391953
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20071115
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 49
IP  - 4
DP  - 2001 Apr
TI  - [Present situation and problems in allergy testing in vitro].
PG  - 381-7
AB  - The present situation and problems related to in vitro assays for measurement of 
      specific immunoglobulin E(IgE) are described. A comparison of four commercially 
      available assay kits(CAP, AlaSTAT, MAST and QAS) revealed that of the allergens 
      studied, common ragweed, mugwort and fungus(aspergillus, candida and alternaria) 
      showed low correlation coefficients. The precise reason for this discordance is 
      not clear, however, we speculated that difference in the origin and extraction 
      method of allergens may affect the correlation results. Random 
      between-the-lot-difference was noted: the results for egg white and milk in QAS 
      showed differences of two classes, although the total imprecision expressed in 
      CV(%) was less than 12% when only a single lot was tested. These results suggest 
      that the specific IgE assay may need an improvement in reagents(approval of 
      standard materials and establishment of standard procedures for allergen 
      extraction) and careful daily quality control by the user.
FAU - Ikeda, M
AU  - Ikeda M
AD  - Department of Clinical Laboratory, Dokkyo University Hospital, Mibu, Tochigi 
      321-0293.
FAU - Ieiri, T
AU  - Ieiri T
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
RN  - 0 (Allergens)
RN  - 0 (Reagent Kits, Diagnostic)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/immunology
MH  - Autoimmune Diseases/*diagnosis/immunology
MH  - Evaluation Studies as Topic
MH  - Humans
MH  - Hypersensitivity/*diagnosis/immunology
MH  - Immunoglobulin E/*blood
MH  - Radioallergosorbent Test
MH  - Reagent Kits, Diagnostic/*standards
MH  - Thyroid Diseases/*diagnosis/immunology
EDAT- 2001/06/08 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/06/08 10:00
PHST- 2001/06/08 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/06/08 10:00 [entrez]
PST - ppublish
SO  - Rinsho Byori. 2001 Apr;49(4):381-7.