PMID- 11393169
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20190607
IS  - 1535-3702 (Print)
IS  - 1535-3699 (Linking)
VI  - 226
IP  - 5
DP  - 2001 May
TI  - Cholangiohepatitis and inflammatory bowel disease induced by a novel 
      urease-negative Helicobacter species in A/J and Tac:ICR:HascidfRF mice.
PG  - 420-8
AB  - Helicobacter bilis and H. hepaticus, both urease-positive intestinal 
      helicobacters of mice, have been shown experimentally to induce proliferative 
      typhlocolitis in scid mice. We recently isolated a urease-negative Helicobacter 
      sp. (H. sp.) that also induced proliferative typhlocolitis in pilot studies in 
      scid mice. To determine the pathogenic potential of H. sp. in immunocompromised 
      and immunocompetent mice, 5-week old male A/J or Tac:Icr:Ha(ICR)-scidfRF mice 
      were inoculated by intraperitoneal (IP) injection with approximately 3 x 10(7) 
      colony-forming units (CFU) of H. sp. Mice were necropsied at various time points 
      postinoculation (PI). Sham-inoculated mice had no clinical, gross, or 
      histopathological lesions. In contrast, scid mice inoculated IP with H. sp. had 
      severe hemorrhagic diarrhea and decreased weight gain at 2, 7, and 18 weeks 
      postinoculation (PI), with severe proliferative typhlocolitis, phlebothrombosis, 
      and hepatitis. A/J mice had no clinical signs, but had mild to moderate 
      proliferative typhlocolitis and moderate to marked cholangiohepatitis at 7 and 24 
      weeks PI. A/J mice infected with H. sp. developed robust immune responses of a 
      predominant Th1 type. This report demonstrates that infection with a 
      urease-negative helicobacter can cause inflammatory bowel disease (IBD) and 
      hepatitis in scid and immunocompetent A/J mice. These results provide a new model 
      of IBD and cholangio-hepatitis associated with a specific urease-negative, novel 
      H. species.
FAU - Shomer, N H
AU  - Shomer NH
AD  - Division of Comparative Medicine, Massachusetts Institute of Technology, 
      Cambridge 02139, USA.
FAU - Dangler, C A
AU  - Dangler CA
FAU - Schrenzel, M D
AU  - Schrenzel MD
FAU - Whary, M T
AU  - Whary MT
FAU - Xu, S
AU  - Xu S
FAU - Feng, Y
AU  - Feng Y
FAU - Paster, B J
AU  - Paster BJ
FAU - Dewhirst, F E
AU  - Dewhirst FE
FAU - Fox, J G
AU  - Fox JG
LA  - eng
GR  - P01CA26731/CA/NCI NIH HHS/United States
GR  - R01CA67529/CA/NCI NIH HHS/United States
GR  - R01DK52413/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin G)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 3.5.1.5 (Urease)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Cholangitis/*etiology/microbiology/pathology
MH  - Helicobacter/enzymology/genetics/immunology/*pathogenicity/ultrastructure
MH  - Helicobacter Infections/*etiology/microbiology/pathology
MH  - Hepatitis A/*etiology/microbiology/pathology
MH  - Immunoglobulin A/biosynthesis
MH  - Immunoglobulin G/blood
MH  - Inflammatory Bowel Diseases/*etiology/microbiology/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred A
MH  - Mice, Inbred ICR
MH  - Mice, SCID
MH  - Microscopy, Electron
MH  - Phylogeny
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Urease/metabolism
EDAT- 2001/06/08 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/06/08 10:00
PHST- 2001/06/08 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/06/08 10:00 [entrez]
AID - 10.1177/153537020122600505 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2001 May;226(5):420-8. doi: 10.1177/153537020122600505.