PMID- 11398813
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20131121
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 14
IP  - 2
DP  - 2001
TI  - The role of interleukin-18 in vesicular stomatitis virus infection of the CNS.
PG  - 181-91
AB  - Intranasal application of vesicular stomatitis virus (VSV) results in the initial 
      infection of the olfactory receptor neurons and a rapid progression of the virus 
      through the mouse central nervous system (CNS). Interleukin-18 (IL-18) is an 
      18.3-kd cytokine that induces interferon gamma (IFN-gamma) production in mice. 
      IL-18 is synthesized as an inactive precursor that is cleaved and activated by 
      caspase-1/interleukin-1beta converting enzyme (ICE). IL-18 shares several 
      biological properties with IL-12, including the ability to induce IFN-gamma 
      production in T lymphocytes and natural killer (NK) cells. In the CNS, microglia 
      and astrocytes produce IL-18 and IL-12. We have previously shown that IL-12 
      promotes recovery from VSV encephalitis. This led us to examine the potential 
      role of IL-18 in the pathogenesis of VSV encephalitis. We show that both IL-18 
      and caspase-1 mRNA are consistently present in the CNS of mice. The addition of 
      exogenous IL-18 to cell cultures does not affect the production of VSV, and 
      addition of exogenous IL-18 at the time of infection does not alter the morbidity 
      or mortality of BALB/c mice. In vitro studies with neutralizing monoclonal 
      antibody to IL-18 had no effect. From these results we conclude that in this 
      system and under the experimental conditions used, unlike IL-12 and IFN-gamma, 
      IL-18 does not play a significant role in the host response to VSV infection.
FAU - Hodges, J L
AU  - Hodges JL
AD  - Department of Biology, New York University, New York 10003-6688, USA.
FAU - Ireland, D D
AU  - Ireland DD
FAU - Reiss, C S
AU  - Reiss CS
LA  - eng
GR  - DC03536/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Interleukin-18)
RN  - 0 (RNA, Messenger)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Caspase 1/genetics
MH  - Central Nervous System Viral Diseases/*etiology
MH  - Immunoblotting
MH  - Interleukin-18/genetics/*physiology/therapeutic use
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neuroblastoma/virology
MH  - Nitric Oxide/biosynthesis
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rhabdoviridae Infections/drug therapy/*etiology/mortality
MH  - Tumor Cells, Cultured
MH  - *Vesicular stomatitis Indiana virus/isolation & purification
EDAT- 2001/06/12 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/06/12 10:00
PHST- 2001/06/12 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/06/12 10:00 [entrez]
AID - 10.1089/088282401750234556 [doi]
PST - ppublish
SO  - Viral Immunol. 2001;14(2):181-91. doi: 10.1089/088282401750234556.