PMID- 11398973
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190901
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 110
IP  - 1
DP  - 2001 Apr
TI  - A tandemly repeated DNA sequence is associated with both knob-like 
      heterochromatin and a highly decondensed structure in the meiotic pachytene 
      chromosomes of rice.
PG  - 24-31
AB  - Highly repetitive tandem DNA sequence repeats are often associated with 
      centromeric and telomeric regions of eukaryotic chromosomes. The rice tandem 
      repeat Os48 is organized as long arrays of a 355 bp monomer and is mainly located 
      in the telomeric regions. The chromosomal locations of the Os48 sequence were 
      determined by fluorescence in situ hybridization (FISH) on rice pachytene 
      chromosomes. The majority of the Os48 loci are associated with brightly 
      4',6-diamidino-2-phenylindole (DAPI)-stained and knob-like heterochromatin in 
      rice pachytene chromosomes. As with other DNA sequences located in the 
      heterochromatic regions, the cytosines of the CG and C(A/T)G sites within the 
      Os48 repeat are heavily methylated. Surprisingly, a proportion of the FISH 
      signals are highly decondensed and deviate significantly from the DAPI-stained 
      periphery of the pachytene chromosomes. This highly decondensed chromatin 
      structure has not been reported in pachytene chromosomes prepared from 
      alcohol/acid-fixed meiotic samples in any other eukaryotic species. The 
      condensation of the Os48 sequences is dynamic during prophase I of meiosis. The 
      FISH signals derived from the Os48 repeat progress from a condensed configuration 
      between leptonema and early pachynema into a decondensed structure from middle 
      pachynema to diakinesis, and then return to a condensed form at metaphase I.
FAU - Cheng, Z
AU  - Cheng Z
AD  - Department of Horticulture, University of Wisconsin-Madison, 53706, USA.
FAU - Stupar, R M
AU  - Stupar RM
FAU - Gu, M
AU  - Gu M
FAU - Jiang, J
AU  - Jiang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA, Plant)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Base Sequence
MH  - Chromosomes/ultrastructure
MH  - DNA Methylation
MH  - DNA, Plant/*genetics
MH  - Genome, Plant
MH  - Heterochromatin/genetics/ultrastructure
MH  - In Situ Hybridization, Fluorescence
MH  - Meiosis
MH  - Oryza/*genetics/ultrastructure
MH  - Physical Chromosome Mapping
MH  - Tandem Repeat Sequences
MH  - Transcription, Genetic
EDAT- 2001/06/12 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/06/12 10:00
PHST- 2001/06/12 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/06/12 10:00 [entrez]
AID - 10.1007/s004120000126 [doi]
PST - ppublish
SO  - Chromosoma. 2001 Apr;110(1):24-31. doi: 10.1007/s004120000126.