PMID- 11399036
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20181130
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 293
IP  - 2
DP  - 2001 Jun 15
TI  - Quantitative detection in the attomole range for immunochromatographic tests by 
      means of a flatbed scanner.
PG  - 224-31
AB  - This work describes the use of the combination of carbon black as an antibody 
      label, a membrane-based immunochromatographic device, and a flatbed scanner as a 
      quantitative test system. The scanner detected 0.4-345 ng carbon black/mm(2) on a 
      nitrocellulose membrane (0.2-170 amol carbon black/mm(2)) with an imprecision 
      (coefficient of variation, CV) lower than 2% for the carbon black determination 
      and a detection limit of 0.04 ng carbon black/mm(2) (0.02 amol/mm(2)). The 
      detection ability was compared to that obtained with alkaline phosphatase (ALP) 
      using a substrate yielding a chemiluminescent signal (0.02 amol ALP/well), 
      beta-galactosidase using a substrate yielding a fluorescent signal (0.3 amol 
      beta-galactosidase/well), and horseradish peroxidase (HRP) using a substrate 
      yielding a colored signal (5 amol HRP/microtiter well). The carbon black 
      immunochromatographic test for immunoglobulin E (IgE) showed a detection limit of 
      0.13 pM IgE (0.01 kU/L) after a testing time of 10 min. The scanner detection 
      imprecision for the IgE determination was 0.6% CV in the range 1-10 kU IgE/L when 
      2.3 mm(2) was used for detection and 1% CV when 0.19 mm(2) was used. A flatbed 
      scanner is an inexpensive instrument with multiple uses, which now also includes 
      the sensitive evaluation of immunoassays.
CI  - Copyright 2001 Academic Press.
FAU - Lonnberg, M
AU  - Lonnberg M
AD  - Center for Surface Biotechnology, Uppsala, S-751 23, Sweden. 
      maria.lonnberg@pharmacia.com
FAU - Carlsson, J
AU  - Carlsson J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-44-0 (Carbon)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Adsorption
MH  - Alkaline Phosphatase/immunology/metabolism
MH  - Carbon/*metabolism
MH  - Chromatography, Affinity/instrumentation/*methods
MH  - Colorimetry
MH  - Electronic Data Processing/*instrumentation/*methods
MH  - Fluorescence
MH  - Horseradish Peroxidase/immunology/metabolism
MH  - Immunoassay/instrumentation/*methods
MH  - Immunoenzyme Techniques/methods
MH  - Immunoglobulin E/analysis/immunology
MH  - Luminescent Measurements
MH  - Molecular Weight
MH  - Protein Binding
MH  - Sensitivity and Specificity
MH  - *Staining and Labeling
MH  - beta-Galactosidase/immunology/metabolism
EDAT- 2001/06/12 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/06/12 10:00
PHST- 2001/06/12 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/06/12 10:00 [entrez]
AID - S0003-2697(01)95130-9 [pii]
AID - 10.1006/abio.2001.5130 [doi]
PST - ppublish
SO  - Anal Biochem. 2001 Jun 15;293(2):224-31. doi: 10.1006/abio.2001.5130.