PMID- 11399096
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20161018
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 9
IP  - 4
DP  - 2001 May
TI  - Differential regulation of gelatinases by transforming growth factor beta-1 in 
      normal equine chondrocytes.
PG  - 325-31
AB  - OBJECTIVE: Cartilage destruction in osteoarthritis (OA) is associated with 
      increased levels of several matrix metalloproteinases (MMPs), including the 
      gelatinases MMP-2 and MMP-9. While increases in some MMPs may be destructive, 
      up-regulation of others may result from increases in normal tissue turnover. The 
      production of MMP-2 and MMP-9 by the anabolic transforming growth factor beta-1 
      (TGF-beta1) in normal equine chondrocytes was investigated. DESIGN: Equine 
      chondrocytes from clinically normal femoropatellar joints were maintained in 
      alginate beads. After serum deprivation, cells were exposed to TGF-beta1 at 
      several concentrations for varying times. Activities of MMP-2 and MMP-9 were 
      estimated by gelatin zymography, while mRNA for MMP-2, MMP-9 and collagen type II 
      were detected using reverse transcription-polymerase chain reaction. RESULTS: 
      Stimulation with TGF-beta1 differentially regulated MMP-2 and MMP-9, with strong 
      up-regulation of both MMP-9 mRNA and enzyme. Increases in MMP-9 enzyme were 
      dose-dependent (0-49 h). There is some evidence suggesting a slight reduction in 
      MMP-2 release following stimulation. Collagen type II mRNA was transiently 
      increased following stimulation. CONCLUSIONS: The different effects of TGF-beta1 
      on MMP-2 and MMP-9 production by normal chondrocytes suggests different roles for 
      these enzymes. The increases in both MMP-9 and collagen type II mRNA following 
      stimulation may suggest a role for MMP-9 in tissue maintenance. Therefore, 
      increased MMP-9 may be secondary to, as distinct from a cause of, cartilage 
      damage.
CI  - Copyright 2001 OsteoArthritis Research Society International.
FAU - Thompson, C C
AU  - Thompson CC
AD  - Connective Tissue Research Group, Faculty of Veterinary Science, University of 
      Liverpool, Liverpool, L69 3BX, UK.
FAU - Clegg, P D
AU  - Clegg PD
FAU - Carter, S D
AU  - Carter SD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Cartilage/*metabolism
MH  - Chondrocytes/*physiology
MH  - Collagen/metabolism
MH  - Hindlimb
MH  - Horses
MH  - Joints
MH  - Matrix Metalloproteinase 2/*biosynthesis
MH  - Matrix Metalloproteinase 9/*biosynthesis
MH  - RNA, Messenger/metabolism
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 2001/06/12 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/06/12 10:00
PHST- 2001/06/12 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/06/12 10:00 [entrez]
AID - S1063-4584(00)90392-3 [pii]
AID - 10.1053/joca.2000.0392 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2001 May;9(4):325-31. doi: 10.1053/joca.2000.0392.