PMID- 11399858
OWN - NLM
STAT- MEDLINE
DCOM- 20010719
LR  - 20171101
IS  - 0009-3157 (Print)
IS  - 0009-3157 (Linking)
VI  - 47
IP  - 4
DP  - 2001 Jul-Aug
TI  - Investigation of oxacillin-hydrolyzing beta-lactamase in borderline 
      methicillin-resistant clinical isolates of Staphylococcus aureus.
PG  - 233-8
AB  - BACKGROUND: Mechanisms of borderline resistance of Staphylococcus aureus to 
      penicillinase-resistant penicillins (PRPs) may include hyperproduction of 
      classical penicillinase and/or production of beta-lactamase hydrolyzing also 
      PRPs. METHODS: beta-Lactamase activity of whole cells and purified enzymes was 
      estimated spectrophotometrically and in isolated cytoplasmic membranes by 
      bioassay with Bacillus subtilis as test strain. RESULTS: Out of 53 clinical 
      isolates of S. aureus, 18 showed oxacillin MIC values from 0.5 to 2 microg/ml, 
      which were reduced by sulbactam and/or clavulanic acid in the case of four 
      isolates producing large quantities of inducible, type A beta-lactamase. 
      Cytoplasmic membranes isolated from these strains showed oxacillin-hydrolyzing 
      activity. One of these strains was grown also in the presence of globomycin, an 
      antibiotic known to interfere with the anchorage of membrane lipoproteins; this 
      treatment eliminated the oxacillin-hydrolyzing activity. CONCLUSIONS: The 
      resistance in these strains was due to a membrane-bound lipoprotein with 
      oxacillin-hydrolyzing activity.
FAU - Gal, Z
AU  - Gal Z
AD  - Department of Pharmacology, Medical and Health Science Center, and Institute of 
      Biochemistry, Faculty of Sciences, University of Debrecen, Debrecen, Hungary.
FAU - Kovacs, P
AU  - Kovacs P
FAU - Hernadi, F
AU  - Hernadi F
FAU - Barabas, G
AU  - Barabas G
FAU - Kiss, L
AU  - Kiss L
FAU - Igloi, A
AU  - Igloi A
FAU - Szabo, I
AU  - Szabo I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Chemotherapy
JT  - Chemotherapy
JID - 0144731
RN  - 0 (Penicillins)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Humans
MH  - Hydrolysis
MH  - Methicillin Resistance/genetics/*physiology
MH  - Oxacillin/*metabolism/pharmacology
MH  - Penicillins/*metabolism/pharmacology
MH  - Staphylococcus aureus/classification/*drug effects/enzymology
MH  - beta-Lactamases/*physiology
EDAT- 2001/06/16 10:00
MHDA- 2001/07/20 10:01
CRDT- 2001/06/16 10:00
PHST- 2001/06/16 10:00 [pubmed]
PHST- 2001/07/20 10:01 [medline]
PHST- 2001/06/16 10:00 [entrez]
AID - 48528 [pii]
AID - 10.1159/000048528 [doi]
PST - ppublish
SO  - Chemotherapy. 2001 Jul-Aug;47(4):233-8. doi: 10.1159/000048528.