PMID- 11399879
OWN - NLM
STAT- MEDLINE
DCOM- 20010830
LR  - 20171101
IS  - 1015-3837 (Print)
IS  - 1015-3837 (Linking)
VI  - 16
IP  - 4
DP  - 2001 Jul-Aug
TI  - Effects of intra-amniotic meconium exposure on the fetal rat: development of a 
      pathogenic model.
PG  - 203-7
AB  - OBJECTIVE: To develop an in vivo animal model for the study of the effects of 
      intrauterine meconium exposure on the fetus. METHODS: Timed pregnant Long-Evans 
      rats were purchased on gestational day (GD) 12 and allowed to acclimate for at 
      least 48 h prior to surgery. Laparotomy was performed and both uterine horns were 
      exteriorized through the abdominal incision. A 26-gauge needle was used to inject 
      either 0.1-cm(3) sterile normal saline or a 20% meconium suspension into each 
      individual gestational sac. The uterus was returned to the abdomen and the 
      incision was closed. On GD 21 (term = 21 days) a cesarean section was completed 
      and the number and viability of fetuses in each horn were recorded. RESULTS: A 
      total of 14 animals were involved in this pilot study. One rat underwent sham 
      surgery with only intra-amniotic saline injection and 13/15 fetuses survived to 
      term. Two animals that underwent surgery on day 18 expired < 24 h postinjection. 
      Eleven maternal animals were injected on GD 20 and underwent cesarean delivery at 
      term; survival rates for saline-injected animals were 71.2% compared to 66.2% for 
      meconium-exposed fetuses. CONCLUSION: We have established an in vivo animal model 
      that allows for the examination of the effects of prolonged intrauterine meconium 
      exposure on the fetus.
CI  - Copyright 2001 S. Karger AG, Basel
FAU - Blackwell, S C
AU  - Blackwell SC
AD  - Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, 
      Wayne State University/Hutzel Hospital, Detroit, Mich., USA. mfmboy@aol.com
FAU - Hallak, M
AU  - Hallak M
FAU - Hotra, J W
AU  - Hotra JW
FAU - Hassan, S S
AU  - Hassan SS
FAU - Berry, S M
AU  - Berry SM
FAU - Sorokin, Y
AU  - Sorokin Y
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Fetal Diagn Ther
JT  - Fetal diagnosis and therapy
JID - 9107463
SB  - IM
MH  - Amniotic Fluid
MH  - Animals
MH  - *Disease Models, Animal
MH  - Female
MH  - Humans
MH  - Infant, Newborn
MH  - Meconium
MH  - Meconium Aspiration Syndrome/*physiopathology
MH  - Pregnancy
MH  - Rats
MH  - *Rats, Long-Evans
MH  - Uterus/surgery
EDAT- 2001/06/16 10:00
MHDA- 2001/08/31 10:01
CRDT- 2001/06/16 10:00
PHST- 2001/06/16 10:00 [pubmed]
PHST- 2001/08/31 10:01 [medline]
PHST- 2001/06/16 10:00 [entrez]
AID - 53910 [pii]
AID - 10.1159/000053910 [doi]
PST - ppublish
SO  - Fetal Diagn Ther. 2001 Jul-Aug;16(4):203-7. doi: 10.1159/000053910.