PMID- 11400155
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20220309
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 194
IP  - 2
DP  - 2001 Jun
TI  - Nerve growth factor and brain-derived neurotrophic factor mRNAs are regulated in 
      distinct cell populations of rat heart after ischaemia and reperfusion.
PG  - 247-53
AB  - Neurotrophins play a crucial role in the development of the peripheral nervous 
      system and their mRNAs are often regulated after several types of tissue injury. 
      This study has investigated the regulation of nerve growth factor (NGF), 
      brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) mRNAs 30 min 
      after myocardial ischaemia followed by reperfusion, by northern blotting, and in 
      situ hybridization in a rat model. Between 2 and 120 h of reperfusion, Ngf mRNA 
      levels showed two- to four-fold up-regulation compared with sham-operated hearts. 
      Scattered Ngf-expressing cells, probably pericytes, were detected in the viable 
      border zone of the myocardium in close association with capillaries, venules, and 
      arterioles. In addition, diffuse Ngf expression was seen in the infarct area 
      after 120 h of reperfusion. Bdnf mRNA showed transient up-regulation after 2 and 
      5 h of reperfusion and remained at control levels thereafter. Bdnf was expressed 
      in the myocytes of the viable border zone. Nt-3 expression showed no significant 
      changes compared with sham-operated hearts. These results suggest a role for NGF 
      and/or BDNF in the pathogenesis of reperfusion injury or in the alterations of 
      cardiac sensory and sympathetic neuronal function after myocardial ischaemia and 
      reperfusion.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Hiltunen, J O
AU  - Hiltunen JO
AD  - Program of Molecular Neurobiology, Institute of Biotechnology, Viikki Biocenter, 
      University of Helsinki, Finland. juhiltun@cc.helsinki.fi
FAU - Laurikainen, A
AU  - Laurikainen A
FAU - Vakeva, A
AU  - Vakeva A
FAU - Meri, S
AU  - Meri S
FAU - Saarma, M
AU  - Saarma M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Northern/methods
MH  - Brain-Derived Neurotrophic Factor/analysis/*genetics
MH  - Immunohistochemistry
MH  - In Situ Hybridization/methods
MH  - Male
MH  - Myocardial Reperfusion Injury/*metabolism
MH  - Myocardium/chemistry/*metabolism
MH  - Nerve Growth Factor/analysis/*genetics
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2001/06/16 10:00
MHDA- 2001/06/22 10:01
CRDT- 2001/06/16 10:00
PHST- 2001/06/16 10:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2001/06/16 10:00 [entrez]
AID - 10.1002/path.878 [pii]
AID - 10.1002/path.878 [doi]
PST - ppublish
SO  - J Pathol. 2001 Jun;194(2):247-53. doi: 10.1002/path.878.