PMID- 11401409
OWN - NLM
STAT- MEDLINE
DCOM- 20010628
LR  - 20161124
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 232
IP  - 2
DP  - 2001 Apr 15
TI  - Epithelial overexpression of BDNF or NT4 disrupts targeting of taste neurons that 
      innervate the anterior tongue.
PG  - 508-21
AB  - Brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4) are essential 
      for the survival of geniculate ganglion neurons, which provide the sensory 
      afferents for taste buds of the anterior tongue and palate. To determine how 
      these target-derived growth factors regulate gustatory development, the taste 
      system was examined in transgenic mice that overexpress BDNF (BDNF-OE) or NT4 
      (NT4-OE) in basal epithelial cells of the tongue. Overexpression of BDNF or NT4 
      caused a 93 and 140% increase, respectively, in the number of geniculate ganglion 
      neurons. Surprisingly, both transgenic lines had severe reduction in fungiform 
      papillae and taste bud number, primarily in the dorsal midregion and ventral tip 
      of the tongue. No alterations were observed in taste buds of circumvallate or 
      incisal papillae. Fungiform papillae were initially present on tongues of newborn 
      BDNF-OE animals, but many were small, poorly innervated, and lost postnatally. To 
      explain the loss of nerve innervation to fungiform papillae, the facial nerve of 
      developing animals was labeled with the lipophilic tracer DiI. In contrast to 
      control mice, in which taste neurons innervated only fungiform papillae, taste 
      neurons in BDNF-OE and NT4-OE mice innervated few fungiform papillae. Instead, 
      some fibers approached but did not penetrate the epithelium and aberrant 
      innervation to filiform papillae was observed. In addition, some papillae that 
      formed in transgenic mice had two taste buds (instead of one) and were frequently 
      arranged in clusters of two or three papillae. These results indicate that 
      target-derived BDNF and NT4 are not only survival factors for geniculate ganglion 
      neurons, but also have important roles in regulating the development and spatial 
      patterning of fungiform papilla and targeting of taste neurons to these sensory 
      structures.
CI  - Copyright 2001 Academic Press.
FAU - Krimm, R F
AU  - Krimm RF
AD  - Department of Pathology and Laboratory Medicine, University of Kentucky Medical 
      Center, Lexington, Kentucky 40536-0298, USA. rkrimm@pop.uky.edu
FAU - Miller, K K
AU  - Miller KK
FAU - Kitzman, P H
AU  - Kitzman PH
FAU - Davis, B M
AU  - Davis BM
FAU - Albers, K M
AU  - Albers KM
LA  - eng
GR  - F32 DC000370/DC/NIDCD NIH HHS/United States
GR  - F32DC00370/DC/NIDCD NIH HHS/United States
GR  - NS33730/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Body Patterning/genetics
MH  - Brain-Derived Neurotrophic Factor/*genetics/*physiology
MH  - Cell Survival
MH  - Embryonic and Fetal Development/genetics
MH  - Epithelium/embryology/growth & development/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Mice
MH  - Mice, Transgenic
MH  - Nerve Growth Factors/*genetics/*physiology
MH  - Neurons/cytology
MH  - Taste Buds/*embryology/*growth & development
MH  - Tongue/embryology/growth & development/innervation
EDAT- 2001/06/13 10:00
MHDA- 2001/06/29 10:01
CRDT- 2001/06/13 10:00
PHST- 2001/06/13 10:00 [pubmed]
PHST- 2001/06/29 10:01 [medline]
PHST- 2001/06/13 10:00 [entrez]
AID - S0012-1606(01)90190-3 [pii]
AID - 10.1006/dbio.2001.0190 [doi]
PST - ppublish
SO  - Dev Biol. 2001 Apr 15;232(2):508-21. doi: 10.1006/dbio.2001.0190.