PMID- 11403379
OWN - NLM
STAT- MEDLINE
DCOM- 20011018
LR  - 20191210
IS  - 0932-0113 (Print)
IS  - 0932-0113 (Linking)
VI  - 87
IP  - 5
DP  - 2001 May
TI  - Inhibition of encystation of Entamoeba invadens by wortmannin.
PG  - 371-5
AB  - Using an axenic encystation system in vitro, we examined the effect of 
      wortmannin, a potent inhibitor of phosphatidylinositol 3-kinase (PI 3-kinase), 
      which is a signaling molecule responsible for numerous cellular responses, on the 
      encystation of Entamoeba invadens. Wortmannin inhibited both encystation and 
      growth of E. invadens strain IP-1 in a dose-dependent manner, the former being 
      more resistant to the drug than the latter. There was little decrease in the 
      number of trophozoites after 3 days of culture in encystation medium containing 
      wortmannin; and the cells remained motile, suggesting that the inhibitory effect 
      of the drug on encystation was not due to its toxic effect on trophozoites. The 
      addition of wortmannin after the induction of encystation was also inhibitory for 
      encystation. Trophozoites incubated for 1 day in encystation medium with 
      wortmannin did not encyst after removal of the drug, suggesting that the drug 
      effect was not reversible in encystation medium. In contrast, trophozoites 
      cultured in growth medium with wortmannin did encyst after their transfer to 
      encystation medium without the drug. Encystation with wortmannin was more 
      strongly inhibited among trophozoites grown in the presence of the drug than 
      among those grown in the absence of the drug. The process of cyst maturation was 
      slightly affected by wortmannin. These results suggest a possible role for PI 
      3-kinase in the signaling involved in the encystation of E. invadens.
FAU - Makioka, A
AU  - Makioka A
AD  - Department of Tropical Medicine, Jikei University School of Medicine, Tokyo, 
      Japan. makioka@jikei.ac.jp
FAU - Kumagai, M
AU  - Kumagai M
FAU - Ohtomo, H
AU  - Ohtomo H
FAU - Kobayashi, S
AU  - Kobayashi S
FAU - Takeuchi, T
AU  - Takeuchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Parasitol Res
JT  - Parasitology research
JID - 8703571
RN  - 0 (Androstadienes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - Androstadienes/*pharmacology
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Entamoeba histolytica/*drug effects/enzymology/*growth & development
MH  - Enzyme Inhibitors/*pharmacology
MH  - In Vitro Techniques
MH  - *Phosphoinositide-3 Kinase Inhibitors
MH  - Signal Transduction/drug effects
MH  - Wortmannin
EDAT- 2001/06/14 10:00
MHDA- 2001/10/19 10:01
CRDT- 2001/06/14 10:00
PHST- 2001/06/14 10:00 [pubmed]
PHST- 2001/10/19 10:01 [medline]
PHST- 2001/06/14 10:00 [entrez]
AID - 10.1007/s004360000339 [doi]
PST - ppublish
SO  - Parasitol Res. 2001 May;87(5):371-5. doi: 10.1007/s004360000339.