PMID- 11403909
OWN - NLM
STAT- MEDLINE
DCOM- 20010927
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 178
IP  - 1-2
DP  - 2001 Jun 10
TI  - Photoperiod-dependent capability of androgen aromatization and the role of 
      estrogens in the bank vole testis visualized by means of immunohistochemistry.
PG  - 189-98
AB  - Detection of steroid hormone receptors within a target tissue is important for an 
      understanding of their crucial role in regulating of steroids' action. In the 
      light of recent knowledge on the role of estrogens in male gonads the efforts 
      were undertaken to clarify and discuss a role of androgen receptors, aromatase 
      and estrogen receptors (ER) in mediating testosterone and/or estradiol action in 
      testicular cells of bank voles that were kept under short or long light cycles. 
      Immunohistochemistry was performed on paraplast embedded sections of the bank 
      vole testes. First, androgen receptors were immunolocalized in testicular somatic 
      cells while germ cell did not express any immunoreaction. Moreover, the ability 
      to convert androgens to estrogens by various testicular cells was documented; 
      aromatase immunoexpression was found in testis sections, not only in Leydig cells 
      and Sertoli cells but also in germ cells. Finally, the expression of estrogen 
      receptor-alpha (ERalpha) was observed in Leydig cells whereas the presence of 
      estrogen receptor-beta (ERbeta) was detected in Sertoli and germ cells, namely 
      spermatocytes and spermatids. The cellular distribution of androgen receptors 
      appeared to be light -and age-dependent in adults; immunoexpression of aromatase 
      and ERbeta was found to be both age -and photoperiod-dependent in germ cells.
FAU - Bilinska, B
AU  - Bilinska B
AD  - Laboratory of Animal Endocrinology and Tissue Culture, Institute of Zoology, 
      Jagiellonian University, Ingardena 6, 30-060, Krakow, Poland. 
      bbili@zuk.iz.uj.edu.pl
FAU - Schmalz-Fraczek, B
AU  - Schmalz-Fraczek B
FAU - Kotula, M
AU  - Kotula M
FAU - Carreau, S
AU  - Carreau S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Androgens)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, Estrogen)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - Androgens/*metabolism
MH  - Animals
MH  - Aromatase/metabolism
MH  - Arvicolinae/*metabolism
MH  - Estrogen Receptor alpha
MH  - Estrogen Receptor beta
MH  - Estrogens/*metabolism
MH  - Immunohistochemistry
MH  - Leydig Cells/metabolism
MH  - Male
MH  - Photoperiod
MH  - Receptors, Androgen/metabolism
MH  - Receptors, Estrogen/metabolism
MH  - Sertoli Cells/metabolism
MH  - Spermatids/metabolism
MH  - Spermatocytes/metabolism
MH  - Testis/*metabolism
EDAT- 2001/06/19 10:00
MHDA- 2001/09/28 10:01
CRDT- 2001/06/19 10:00
PHST- 2001/06/19 10:00 [pubmed]
PHST- 2001/09/28 10:01 [medline]
PHST- 2001/06/19 10:00 [entrez]
AID - S0303720701004270 [pii]
AID - 10.1016/s0303-7207(01)00427-0 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2001 Jun 10;178(1-2):189-98. doi: 
      10.1016/s0303-7207(01)00427-0.