PMID- 11404229
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20171213
IS  - 0193-1849 (Print)
IS  - 0193-1849 (Linking)
VI  - 281
IP  - 1
DP  - 2001 Jul
TI  - Stimulatory effects of leptin and muscle contraction on fatty acid metabolism are 
      not additive.
PG  - E122-9
AB  - Leptin has been shown to acutely stimulate fatty acid oxidation and 
      triacylglycerol hydrolysis in skeletal muscle. These effects are similar to those 
      induced by muscle contraction alone. Several studies have demonstrated that, 
      during aerobic exercise, plasma leptin concentrations are well maintained; 
      however, none has examined whether the stimulatory effects of leptin and 
      contraction on muscle lipid metabolism are additive. This is the first study to 
      examine the direct effect of leptin on lipid and carbohydrate (CHO) metabolism in 
      isolated oxidative muscle over a range of contraction intensities. We examined 
      the effect of leptin (10 microg/ml) on the synthesis and degradation of muscle 
      lipid pools [phospholipid (PL), diacylglycerol (DG), triacylglycerol (TG)] and 
      palmitate oxidation in isolated resting and contracting (2, 8, and 20 tetani/min) 
      soleus muscles. At rest, leptin increased fatty acid oxidation (+ 40%, P < 0.05) 
      and TG hydrolysis (+ 47%, P < 0.05), while blunting TG esterification (-20%, P < 
      0.05). Glucose oxidation was unaffected at rest in the presence of leptin. During 
      tetanic contraction, fatty acid oxidation (+20-114%, P < 0.05) and TG 
      esterification (+ 19-33%, P < 0.05) as well as net TG utilization (+ 23%, P < 
      0.05) were all significantly increased. However, leptin was without further 
      effect on any of these parameters during contraction. Net utilization of 
      intramuscular glycogen, as well as glucose oxidation, was unaffected during 
      contraction by leptin. The findings of the present study indicate that leptin has 
      an important influence on lipid metabolism in resting muscle, but not during 
      contraction.
FAU - Lau, R
AU  - Lau R
AD  - Department of Human Biology and Nutritional Sciences, University of Guelph, 
      Guelph N1G 2W1, Canada N2L 3G1.
FAU - Blinn, W D
AU  - Blinn WD
FAU - Bonen, A
AU  - Bonen A
FAU - Dyck, D J
AU  - Dyck DJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol Endocrinol Metab
JT  - American journal of physiology. Endocrinology and metabolism
JID - 100901226
RN  - 0 (Fatty Acids)
RN  - 0 (Leptin)
RN  - 0 (Palmitates)
RN  - 0 (Triglycerides)
SB  - IM
MH  - Animals
MH  - Carbohydrate Metabolism
MH  - Fatty Acids/*metabolism
MH  - Female
MH  - In Vitro Techniques
MH  - Leptin/*pharmacology
MH  - Lipid Metabolism
MH  - Muscle Contraction/*physiology
MH  - Muscle, Skeletal/drug effects/*metabolism
MH  - Oxidation-Reduction
MH  - Palmitates/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stimulation, Chemical
MH  - Triglycerides/metabolism
EDAT- 2001/06/19 10:00
MHDA- 2001/07/13 10:01
CRDT- 2001/06/19 10:00
PHST- 2001/06/19 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/06/19 10:00 [entrez]
AID - 10.1152/ajpendo.2001.281.1.E122 [doi]
PST - ppublish
SO  - Am J Physiol Endocrinol Metab. 2001 Jul;281(1):E122-9. doi: 
      10.1152/ajpendo.2001.281.1.E122.