PMID- 11404381
OWN - NLM
STAT- MEDLINE
DCOM- 20010802
LR  - 20171116
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 69
IP  - 6
DP  - 2001 Jun
TI  - CD8alpha(-) and CD8alpha(+) subclasses of dendritic cells undergo phenotypic and 
      functional maturation in vitro and in vivo.
PG  - 951-8
AB  - Dendritic cells (DCs) are essential for the priming of immune responses. This 
      antigen-presenting function of DCs develops in sequence in a process called 
      maturation, during which they become potent sensitizers of naive T cells but 
      reduce their ability to capture and process antigens. Some heterogeneity exists 
      in mouse-DC populations, and two distinct subsets of DCs expressing high levels 
      of CD11c can be identified on the basis of CD8alpha expression. We have studied 
      the phenotype and maturation state of mouse splenic CD8alpha(-) and CD8alpha(+) 
      DCs. Both subsets were found to reside in the spleen as immature cells and to 
      undergo a phenotypic maturation upon culture in vitro in GM-CSF-containing medium 
      or in vivo in response to lipopolysaccharide. In vitro and in vivo analyses 
      showed that this maturation process is an absolute requisite for DCs to acquire 
      their T-cell priming capacity, transforming CD8alpha(-) and CD8alpha(+) DCs into 
      potent and equally efficient activators of naive CD4(+) and CD8(+) T cells. 
      Furthermore, these results highlight the importance that environmental factors 
      may have on the ability of DC subsets to influence Th responses qualitatively; 
      i.e., the ability to drive Th1 versus Th2 differentiation may not be fixed 
      immutably for each DC subset.
FAU - De Smedt, T
AU  - De Smedt T
AD  - Department of Discovery Research, Immunex Corporation, Seattle, Washington, USA. 
      desmedtt@immunex.com
FAU - Butz, E
AU  - Butz E
FAU - Smith, J
AU  - Smith J
FAU - Maldonado-Lopez, R
AU  - Maldonado-Lopez R
FAU - Pajak, B
AU  - Pajak B
FAU - Moser, M
AU  - Moser M
FAU - Maliszewski, C
AU  - Maliszewski C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8alpha antigen)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (Membrane Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (flt3 ligand protein)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Antigen Presentation
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8 Antigens/*analysis
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured/drug effects
MH  - Dendritic Cells/classification/*cytology/drug effects
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Histocompatibility Antigens Class II/immunology
MH  - Immunophenotyping
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation
MH  - Macrophage-1 Antigen/analysis
MH  - Membrane Proteins/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Ovalbumin/immunology
MH  - Recombinant Proteins/pharmacology
MH  - Spleen/cytology/immunology
EDAT- 2001/06/19 10:00
MHDA- 2001/08/03 10:01
CRDT- 2001/06/19 10:00
PHST- 2001/06/19 10:00 [pubmed]
PHST- 2001/08/03 10:01 [medline]
PHST- 2001/06/19 10:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2001 Jun;69(6):951-8.