PMID- 11404410
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20181113
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 21
IP  - 12
DP  - 2001 Jun 15
TI  - BDNF enhances quantal neurotransmitter release and increases the number of docked 
      vesicles at the active zones of hippocampal excitatory synapses.
PG  - 4249-58
AB  - Brain-derived neurotrophic factor (BDNF) is emerging as a key mediator of 
      activity-dependent modifications of synaptic strength in the CNS. We investigated 
      the hypothesis that BDNF enhances quantal neurotransmitter release by modulating 
      the distribution of synaptic vesicles within presynaptic terminals using 
      organotypic slice cultures of postnatal rat hippocampus. BDNF specifically 
      increased the number of docked vesicles at the active zone of excitatory synapses 
      on CA1 dendritic spines, with only a small increase in active zone size. In 
      agreement with the hypothesis that an increased docked vesicle density enhances 
      quantal neurotransmitter release, BDNF increased the frequency, but not the 
      amplitude, of AMPA receptor-mediated miniature EPSCs (mEPSCs) recorded from CA1 
      pyramidal neurons in hippocampal slices. Synapse number, independently estimated 
      from dendritic spine density and electron microscopy measurements, was also 
      increased after BDNF treatment, indicating that the actions of BNDF on mEPSC 
      frequency can be partially attributed to an increased synaptic density. Our 
      results further suggest that all these actions were mediated via tyrosine kinase 
      B (TrkB) receptor activation, established by inhibition of plasma membrane 
      tyrosine kinases with K-252a. These results provide additional evidence of a 
      fundamental role of the BDNF-TrkB signaling cascade in synaptic transmission, as 
      well as in cellular models of hippocampus-dependent learning and memory.
FAU - Tyler, W J
AU  - Tyler WJ
AD  - Department of Neurobiology, University of Alabama at Birmingham, Birmingham, 
      Alabama 35294-0021, USA.
FAU - Pozzo-Miller, L D
AU  - Pozzo-Miller LD
LA  - eng
GR  - R01-NS40593-01/NS/NINDS NIH HHS/United States
GR  - R01 NS040593/NS/NINDS NIH HHS/United States
GR  - P01 HD038760/HD/NICHD NIH HHS/United States
GR  - P01-HD38760/HD/NICHD NIH HHS/United States
GR  - R01 NS040593-09/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Receptors, AMPA)
RN  - 77521-29-0 (alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Dendrites/metabolism/ultrastructure
MH  - Enzyme Inhibitors/pharmacology
MH  - Excitatory Postsynaptic Potentials/drug effects
MH  - Hippocampus/cytology/*metabolism
MH  - In Vitro Techniques
MH  - Neurotransmitter Agents/*metabolism
MH  - Presynaptic Terminals/*metabolism
MH  - Pyramidal Cells/drug effects/metabolism/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/antagonists & inhibitors/metabolism
MH  - Receptors, AMPA/metabolism
MH  - Signal Transduction/drug effects/physiology
MH  - Synapses/drug effects/*metabolism/ultrastructure
MH  - Synaptic Vesicles/*metabolism/ultrastructure
MH  - alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/metabolism/pharmacology
PMC - PMC2806848
MID - NIHMS166551
EDAT- 2001/06/19 10:00
MHDA- 2001/07/13 10:01
PMCR- 2001/12/15
CRDT- 2001/06/19 10:00
PHST- 2001/06/19 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/06/19 10:00 [entrez]
PHST- 2001/12/15 00:00 [pmc-release]
AID - 21/12/4249 [pii]
AID - 5297 [pii]
AID - 10.1523/JNEUROSCI.21-12-04249.2001 [doi]
PST - ppublish
SO  - J Neurosci. 2001 Jun 15;21(12):4249-58. doi: 10.1523/JNEUROSCI.21-12-04249.2001.