PMID- 11404434
OWN - NLM
STAT- MEDLINE
DCOM- 20010712
LR  - 20240420
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 21
IP  - 12
DP  - 2001 Jun 15
TI  - Brain-derived neurotrophic factor is released in the dorsal horn by distinctive 
      patterns of afferent fiber stimulation.
PG  - 4469-77
AB  - Brain-derived neurotrophic factor (BDNF) is synthesized by small neuron cell 
      bodies in the dorsal root ganglia (DRG) and is anterogradely transported to 
      primary afferent terminals in the dorsal horn where it is involved in the 
      modulation of painful stimuli. Here we show that BDNF is released in the rat 
      isolated dorsal horn after chemical stimulation by capsaicin or electrical 
      stimulation of dorsal roots. Capsaicin superfusion (1-100 microm) induced a 
      dose-dependent release of BDNF, measured using ELISA. The highest dose of 
      capsaicin also induced a depletion of BDNF protein in the dorsal horn. BDNF 
      release was also seen after electrical stimulation of the dorsal roots at C-fiber 
      strength. This release was encoded by specific patterns of afferent fiber 
      stimulation. Neither continuous low-frequency (480 pulses, 1 Hz) nor tetanic 
      high-frequency (300 pulses in 3 trains, 100 Hz) stimulation evoked release of 
      BDNF, although substance P (SP) release was observed under both of these 
      conditions. However, BDNF was released after short bursts of high-frequency 
      stimulation (300 pulses in 75 trains, 100 Hz) along with SP and glutamate. The 
      NMDA antagonist d-AP-5 inhibited electrically evoked BDNF release. BDNF release 
      was also measured after systemic or intrathecal NGF treatment. This upregulated 
      BDNF content in the DRG and increased the capsaicin-evoked release of BDNF. 
      Similarly, the amount of BDNF released by burst stimulation was increased after 
      NGF treatment. This activity-dependent release continued to be encoded solely by 
      this stimulation pattern. These experiments demonstrate that BDNF release in the 
      dorsal horn is encoded by specific patterns of afferent fiber stimulation and is 
      mediated by NMDA receptor activation.
FAU - Lever, I J
AU  - Lever IJ
AD  - Neuroscience Research Center and Department of Pharmacology, Guy's, King's, and 
      St. Thomas' School of Biomedical Sciences, King's College London, London SE1 1UL, 
      United Kingdom.
FAU - Bradbury, E J
AU  - Bradbury EJ
FAU - Cunningham, J R
AU  - Cunningham JR
FAU - Adelson, D W
AU  - Adelson DW
FAU - Jones, M G
AU  - Jones MG
FAU - McMahon, S B
AU  - McMahon SB
FAU - Marvizon, J C
AU  - Marvizon JC
FAU - Malcangio, M
AU  - Malcangio M
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 33507-63-0 (Substance P)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Capsaicin/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Electric Stimulation/methods
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Excitatory Amino Acid Antagonists/pharmacology
MH  - Glutamic Acid/metabolism
MH  - In Vitro Techniques
MH  - Male
MH  - Nerve Growth Factor/pharmacology
MH  - Neurons, Afferent/*physiology
MH  - Posterior Horn Cells/drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
MH  - Spinal Cord/drug effects/*metabolism
MH  - Spinal Nerve Roots/physiology
MH  - Stimulation, Chemical
MH  - Substance P/metabolism
PMC - PMC6762751
EDAT- 2001/06/16 10:00
MHDA- 2001/07/13 10:01
PMCR- 2001/12/15
CRDT- 2001/06/16 10:00
PHST- 2001/06/16 10:00 [pubmed]
PHST- 2001/07/13 10:01 [medline]
PHST- 2001/06/16 10:00 [entrez]
PHST- 2001/12/15 00:00 [pmc-release]
AID - 21/12/4469 [pii]
AID - 5310 [pii]
AID - 10.1523/JNEUROSCI.21-12-04469.2001 [doi]
PST - ppublish
SO  - J Neurosci. 2001 Jun 15;21(12):4469-77. doi: 10.1523/JNEUROSCI.21-12-04469.2001.