PMID- 11404844
OWN - NLM
STAT- MEDLINE
DCOM- 20010809
LR  - 20131121
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 15
IP  - 13
DP  - 2001
TI  - Evaluation of several solid phase extraction liquid chromatography/tandem mass 
      spectrometry on-line configurations for high-throughput analysis of acidic and 
      basic drugs in rat plasma.
PG  - 1075-83
AB  - Several configurations using 6- and 10-port switching valves were studied for 
      high flow, on-line extraction of rat plasma coupled to an electrospray triple 
      quadrupole mass spectrometer. Each plasma sample was diluted 1:1 with an aqueous 
      internal standard solution. The sample was injected into a 2.1 x 20 mm cartridge 
      column packed with 25 microm divinylbenzene/N-vinylpyrrolidone packing using 100% 
      aqueous mobile phase at 4 mL/min. After sample loading and sample cleanup, the 
      analytes were eluted from the extraction column with a 1.0-min gradient at 0.4 
      mL/min. The samples were either analyzed directly after elution from the 
      extraction column or after additional separation using a short high performance 
      liquid chromatography (HPLC) column. The different configurations were tested 
      using an acidic drug (diflunisal) and a basic drug (clemastine) in rat plasma. 
      On-line analysis was performed by injecting 200 microL of diluted plasma. The 
      mass spectrometer was operated in the multiple reaction monitoring (MRM) mode. 
      All calibration standards gave relative standard deviations (RSDs) below 5%. The 
      total time per sample was 3 min.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Mallet, C R
AU  - Mallet CR
AD  - Waters Corporations, 34 Maple St., Milford, MA 01757-3696, USA. 
      claude_mallet@waters.com
FAU - Mazzeo, J R
AU  - Mazzeo JR
FAU - Neue, U
AU  - Neue U
LA  - eng
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Indicators and Reagents)
RN  - 4U5MP5IUD8 (Niflumic Acid)
RN  - 7C546U4DEN (Diflunisal)
RN  - 95QN29S1ID (Clemastine)
RN  - DAA13NKG2Q (Papaverine)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/instrumentation/methods
MH  - Clemastine/*blood
MH  - Diflunisal/*blood
MH  - Equipment Design
MH  - Indicators and Reagents
MH  - Niflumic Acid/*blood
MH  - Online Systems
MH  - Papaverine/*blood
MH  - Rats
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/instrumentation/methods
EDAT- 2001/06/19 10:00
MHDA- 2001/08/10 10:01
CRDT- 2001/06/19 10:00
PHST- 2001/06/19 10:00 [pubmed]
PHST- 2001/08/10 10:01 [medline]
PHST- 2001/06/19 10:00 [entrez]
AID - 10.1002/rcm.346 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2001;15(13):1075-83. doi: 10.1002/rcm.346.